利用均匀设计法优化鸡(土从)母种培养基

Optimum of Stock Culture Medium of Termitomyces spp. by Orthogonal Design

鸡(土从)菌丝生长缓慢,在培养过程中极易被污染,为了提高鸡地从菌丝生长速度,减少被污染的机率,在前期单因素试验筛选鸡(土从)母种培养基中的碳源、氮源、氨基酸、维生素、无机盐、蚁穴浸提物、抑菌剂等结果的基础上,选取马铃薯汁、麦芽糖、酵母膏、VB_9、脯氨酸、可溶性碳酸钙清液、磷酸二氢钾、硫酸镁、蚁穴浸液、链霉素等10个因子,每个因子设12个水平,采用U_(12)(12^(10))均匀设计表设计试验方案,对鸡(土从)母种培养基进行优化。最优母种培养基配方为马铃薯汁126.20 mL·L^(-1)、麦芽糖11.63 g·L^(-1)、酵母膏5.50 g·L^(-1)、VB_9 47.90μg·L^(-1)、脯氨酸23.37μg·L^(-1)、可溶性碳酸钙清液0、磷酸二氢钾0.07 g·L^(-1)、硫酸镁4.19 g·L^(-1)、蚁穴浸提物61.82 mL·L^(-1)、链霉素2.33 mg·L^(-1)。利用该优化配方的培养基培养的鸡(土从)母种,其菌丝生长速度快,长势强。

It’s widely acknowledged that the growth rate of Termitomyces spp.mycelium grows slowly and is very easy to be contaminated during the process of cultivation.In order to improve the mycelium growth rate,as well as reduce the probability of contamination,ten factors including potato juice,maltose,yeast extract,VB9,proline,calcium carbonate solution,potassium dihydrogen phosphate,magnesium sulfate,leaching objects of ant nest and streptomycin were chosen to optimize the stock culture medium of Termitomyce spp.,by using U12(1210)orthogonal design with 12 levels for each factor,which was based on the results of the selected optimal carbon source,nitrogen source,amino acid,vitamin,inorganic salt,leaching objects of ant nest and bacteriostatic agent by single factor experiment in the early stage.Based on the data and results demonstrated,the optimum stock culture medium was potato juice 126.20 mL·L-1,maltose 11.63 g·L-1,yeast extract 5.50 g·L-1,VB9 47.90μg·L-1,proline 23.37μg·L-1,calcium carbonate solution 0,potassium dihydrogen phosphate 0.07 g·L-1,magnesium sulfate 4.19 g·L-1,leaching objects of ant nest 61.82 mL·L-1and streptomycin 2.33 mg·L-1.The mycelium of Termitomyces spp.grew rapidly and vigorously in the medium with the optimized formula.