汉防己甲素对人神经母细胞瘤SH-SY5Y增殖和凋亡的影响

Effects of Tetrandrine on the Proliferation and Apoptosis of Human SH-SY5Y Cells

目的探讨汉防己甲素对神经母细胞瘤SH-SY5Y细胞株的增殖抑制和诱导凋亡的作用。方法通过CCK-8实验检测汉防己甲素对于细胞生长的抑制率,流式细胞术检测Tet作用于细胞后,对细胞活性氧、线粒体膜电位、凋亡和周期的影响。结果汉防己甲素能显著抑制SH-SY5Y细胞生长,基本呈时间-剂量依赖性。流式细胞术检测显示不同浓度汉防己甲素处理后,活性氧明显升高,线粒体膜电位明显下降,细胞凋亡比例明显升高,G_0/G_1期细胞占比明显增高。结论汉防己甲素能够抑制SH-SY5Y细胞增殖并且诱导其发生凋亡。

Objective To investigate the effects of Tetrandrine on the proliferation and apotosis of human SH-SY5 Y cells.Methods CCK-8 assay was used for the cell viability test. Flow cytometry was used to determine the intracellular reactive oxygen species(ROS), mitochondrial membrane potential(JC-1), apoptotic ratio and cell cycle after Tetrandrine treatment. The cells with sham treatment served as the control. Results Tetrandrine significantly inhibited the proliferation of SH-SY5 Y cells in a time-and dose-dependent manner. The cell apoptosis and the production of intracellular ROS also increased remarkably by Tetrandrine treatment while JC-1 decreased. Cell cycle analysis further revealed that the percentage of cells in G0/G1 phase increased, in contrasting a statistical decrease of cell in G2/M phase. Conclusion Tetrandrine could inhibit the proliferation and induce the apoptosis of SH-SY5 Y cells by blocking cell cycle in G0/G1 phase.