不同激素对油用牡丹上胚轴萌发的影响

Effect of Different Hormones on Epicotyl Germination of Oil-used Peony

以‘凤丹白’胚根大于3cm的种子为试验材料,采用全因子试验方法,研究了GA3、NAA和6-BA 3种激素的不同浓度和时间对胚根种子的萌芽率、发芽指数和初萌期的影响,以期为苗木生产提供依据。结果表明:GA3500mg·L^(-1)处理16h,其萌芽率、发芽指数和初萌期分别为98.89%、0.28和77.00d,发芽指数显著高于对照、初萌期显著比对照提早了13.33d;NAA 100mg·L^(-1)和6-BA 100mg·L^(-1)对‘凤丹白’上胚轴萌发均没有明显的促进或抑制作用,300mg·L^(-1) NAA和6-BA、500mg·L^(-1) NAA和6-BA不论浸种时间长短均明显地抑制了‘凤丹白’种子的萌芽率和发芽指数;同时,500mg·L^(-1)NAA显著地延长了‘凤丹白’种子的初萌期,说明500mg·L^(-1) NAA严重抑制了‘凤丹白’种子的生长发育。

To improve the germination rate and germination regularity of Paeonia ostii ‘Feng Dan Bai’and provide theoretical basis for seedling production,the effects of different densities and treated time of 3hormones(GA3,NAA and 6-BA respectively)on germination rate,germination index and first sprouting day of seeds of Paeonia ostii ‘Feng Dan Bai’with radicle length greater than 3cm were studied using full factor test method.The results showed that germination rate,germination index and first sprouting day of the seeds treated for 16 hours in 500 mg·L-1 GA3 were 98.89%,0.28and77.00 days respectively.The germination index was significantly higher than that of control experiment,and the first sprouting day arrived 13.33 days earlier than the control.However,there were no significant effects of NAA and 6-BA at low concentration 100 mg·L^-1 on the hypocotyl growth of Paeonia ostii ‘Feng Dan Bai’.The two treatments of 300 mg·L^-1 NAA and 6-BA treatment,500 mg·L^-1 NAA and 6-BA treatment both had significantly inhibitory effects on the germination rate and germination index of Paeonia ostii ‘Feng Dan Bai’,regardless of the duration of seed soaking.Meanwhile,the 500mg·L^-1 NAA treatment significantly extended the first sprouting day,suggesting that the growth of Paeonia ostii seeds were seriously restrained by 500mg·L^-1 NAA treatment.