脱氧雪腐镰刀菌烯醇暴露对BeWo细胞炎症与凋亡相关基因表达的影响

Deoxynivalenol induced mRNA expressions of inflammation and apoptosis in BeWo cells

目的观察脱氧雪腐镰刀菌烯醇(deoxynivalenol,DON)暴露对人胎盘绒毛癌细胞(BeWo细胞)炎症与凋亡相关基因表达的影响。方法将BeWo细胞进行培养和相应剂量(0～750 nmol/L)DON暴露,ELISA法检测人绒毛膜促性腺激素(β-HCG)水平,qPCR法检测基因白细胞介素(IL)-6、IL-8、肿瘤坏死因子(TNF)-α、环氧酶(Cox)-2、半胱氨酸天冬氨酸蛋白酶(Caspase)-3和B淋巴细胞瘤(Bcl)-xl的表达水平。结果与空白对照组相比,50 nmol/L DON暴露组炎症和凋亡相关基因的表达呈显著性改变,即炎症相关基因IL-6、TNF-α和Cox-2在3、12和24 h时表达均显著上调(P<0.01),而IL-8仅在暴露24 h时显著上调(P<0.01),而凋亡相关基因Caspase-3在3、12和24 h时表达均显著低于对照组(P<0.01),而Bcl-xl的表达在暴露24 h时显著高于对照组(P<0.01)。结论 BeWo细胞DON暴露时炎症反应增强,凋亡被抑制,推测以上结果可能是DON胚胎发育毒性的潜在分子机制之一。

OBJECTIVE To explore the effects of deoxynivalenol-induced mRNA expressions of inflammation and apoptosis in BeWo cells.METHODS After been cultured and transferred,BeWo cells were seeded for the CCK-8 assay.Then incubated with DON in the'appropriate doses and time according to the CCK-8 result.ELISA kit was used to measure the 13-HCG levels in BeWo ceils,qPCR was used to measure mRNA expressions of inflammation (IL-6,IL-8,TNF-α and Cox-2)and apoptosis (Caspase-3 and Bcl-xl)in BeWo ceils.RESULTS Compared with the control group,IL-6,TNF-α and Cox-2 significantly increased in BeWo cells which were exposed in 50 nmol/L DON in 3, 12 and 24 h (P<0.01),while IL-8 only significantly increased in 24 h (P<0.01 ). Caspase-3 significantly decreased in 3,12 and 24 h (P<0.01),and Bcl-xl significantly increased in 24 h (P<0.01).CONCLUSION The change in the level of inflammation and apoptosis may be one of the critical molecular mechanisms for DON-induced embryotoxicity.