慈竹BeCesA1和BeCesA7基因的克隆及组织表达分析

Cloning and Tissue Expression Analysis of BeCesA1 and BeCesA7 in Bambusa emeiensis

植物的纤维素合成酶(CesA)是纤维素合成途径的关键酶,不仅与纤维素含量相关,也与植物生长发育相关。但有关慈竹CesA基因的克隆与分析,以及GA3对慈竹CesA基因表达的影响未见报道。本研究通过慈竹转录组数据库、在线NCBI基因组数据库及毛竹基因组数据库,同源克隆得到两个CesA基因,BeCesA1(GenBank注册号:KY435488)和BeCesA7 (GenBank注册号:KY435489),分别编码1 078和1 053个氨基酸残基。保守结构域分析及多重序列比对分析表明,慈竹BeCesA1和BeCesA7蛋白均在N端存在一个RING finger结构和两个高度可变区域。组织表达结果表明,BeCesA1和BeCesA7两个基因在竹不同组织中表达模式各不相同,但均在100 cm笋等组织中高表达,然而BeCesA7的表达量明显高于BeCesA1。在竹的发育过程中,BeCesA1基因随着笋的发育表达量逐渐降低,而BeCesA7则随着笋的发育逐渐增高。外源施加GA3后,以BeCesA1的CK未展开叶为参照,BeCesA1在茎秆中的表达量降低,而BeCesA7则在茎秆中的表达量增加。

Cellulose synthase(CesA) plays a key role in cellulose biosynthesis, which is not only related to cellulose content, but also involved in plant growth and development. However, the cloning and analysis of CesA gene from Bambusa emeiensis as well as the effect of GA3 on the expression of CesA gene in Bambusa emeiensis have not been reported. In this paper, two CesA genes, BeCesA1(GenBank accession number: KY435488) and BeCes A7(GenBank accession number: KY435489), which encoding 1 078 and 1 053 amino acid residues, respectively,were obtained based on GenBank transcriptome database, online NCBI genomic database and Phytomania genomic database. Conserved domain analysis and multiple sequence alignment analysis indicated that both the BeCesA1 and BeCesA7 proteins had a RING finger structure and two highly variable regions at the N-terminus. Tissue expression analysis showed that the expression patterns of BeCesA1 and BeCesA7 genes differed from each other in various bamboo tissues. Whereas, BeCesA1 and BeCesA7 both expressed highly in the 100 cm bamboo shoot,while the expression of BeCesA7 was higher than BeCesA1. During bamboo development, the expression of BeCes A1 gene gradually decreased with the development of shoots, which was on the contrary with that of BeCesA7.After the exogenous application of GA3, the expression of BeCesA1 in the stalks decreased while that of BeCesA7 increased in the stalks with the unexpanded leaf of BeCesA1 as reference.