摘要
本试验以6月龄、1周岁和2周岁的威宁绵羊公母羊为研究对象,利用分子手段对威宁绵羊GHR基因编码区序列进行克隆,并进行生物信息学分析,此外,本试验同时采用实时荧光定量PCR的手段对GHR基因在威宁绵羊不同性别及不同生长阶段心脏、肝脏、脾脏、肺脏、肾脏及背最长肌6个组织中mRNA水平上的表达规律进行探究,结果显示:威宁绵羊GHR基因CDS区序列长度为1 905 bp,发现2个SNP位点。GHR基因在威宁绵羊各组织均有不同程度的表达,在不同性别、不同生长阶段相同组织中的表达具有一定的显著性差异。
This study was conducted on male and female Weining sheep aged 6 months, 1 year and 2 years,Then cloning GHR gene coding region sequence of Weining sheep, and bioinformatic analysis was carried out. In addition, the experiment used real-time qPCR method to analyze m RNA expression rule of GHR gene in heart,liver, spleen, lung, kidney and longissimus Dorsi of Weining sheep at different growth stages and different gender.The results showed: the sequence length of CDS region of GHR gene in Weining sheep was 1 905 bp, and 2 SNPs loci were found. The result showed that GHR gene was expressed in different degrees in all tissues of Weining sheep and there were significant differences in the expression of different gender and different growth stages in the same tissues.
作者
吴雪
易鸣
程朝友
付正仙
杨德文
程均华
王振
王金洲
刘若余
Wu Xue;Yi Ming;Cheng Chaoyou;Fu Zhengxian;Yang Dewen;Cheng Junhua;Wang zhen;Wang Jinzhou;Liu Ruoyu(College of Animal Sciences/Key Laboratory of Animal Genetics,Breeding and Reproduction in We Plateau Mountain Region,Ministry of Education, Guizhou University,Guiyang,550025;Weining Improvement Station of Livestock and Poultry Variety,Weining,553100;Weining Bureau of Animal Husbandry Industry,Weining,553100)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2019年第1期1-6,共6页
Genomics and Applied Biology
基金
2013年地方畜禽品种保护项目贵州省农委基金项目资助
