乳腺癌中β2肾上腺素受体的表达及其与HER2、K-ras基因的相关性研究

Correlation between expression of β2 adrenergic receptors and HER2, K-ras genes in breast cancer

目的研究乳腺浸润性导管癌组织中β2肾上腺素受体(β2-AR)蛋白的表达情况及其与人类表皮生长因子受体2(HER2)、K-ras基因在乳腺癌中的表达相关性。方法采用免疫组织化学染色链霉素抗生物素蛋白、过氧化物酶法(SP法)检测10例正常乳腺组织、33例乳腺浸润性导管癌石蜡标本中β2肾上腺素受体、HER2、K-ras基因蛋白的表达情况。实验数据采用SPSS17.0统计软件和非参数秩和检验(Mann-Whitney检验)、Spearman等级相关检验统计学方法进行处理和分析。结果β2-AR在所有检测的乳腺癌细胞中都存在表达,过表达率21/33;HER2在乳腺癌细胞中的阳性表达为23/33,过表达率为20/33;K-ras在乳腺癌组织中呈阳性表达(28/33),过表达率为11/33,3者且均明显高于正常乳腺组织(β2-AR的表达Z值=-4.876,P=0.000。HER2的表达Z值=-2.752,P=0.006。K-ras的表达Z值=-3.126,P=0.020)。在对癌组织中β2-AR与HER2、K-ras表达的相关性研究过程中,β2-AR和HER2的表达及β2-AR和K-ras的表达均有相关性。在临床病理参数中,β2-AR的表达仅在有无淋巴结转移组间的差异有统计学意义,在年龄、肿瘤大小、临床分期、病理分级及激素水平的各组中表达差异无统计学意义。结论β2-AR、HER2、K-ras的在乳腺癌组织中的高表达均与乳腺癌发生、发展有关。β2-AR高表达的乳腺癌中,淋巴结潜在转移的风险也较大。β2-AR在乳腺癌发病过程中可能通过HER2和K-ras发挥作用。

Objective To investigate the expression of β2 adrenergic receptor (β2 AR) in invasive ductal carcinoma of the breast, and their correlation with HER2 and K-ras genes in breast cancer. Methods The expression of β2 AR, HER2 and K-ras gene in 10 cases of normal breast tissues and 33 cases of invasive ductal carcinoma of the breast were detected by immunohistochemical staining of streptomycin antibiotin protein and peroxidase assay (SP) . Such statistical methods as SPSS17.0 statistical software, Mann-Whitney test and Spearman rank correlation test were applied to process and analyze the experimental data. Results β2 AR was expressed in all breast cancer cells, with an overexpression rate of 21/33. The positive expression rate of HER2 in breast cancer cells was 23/33, with an overexpression rate of 20/33. K-ras was positively expressed in breast cancer tissue (28/33) , with an overexpression rate of 11/33. All of these three were significantly higher than those in the normal breast tissues. Z value of β-2 AR expression was 4.876, P value was 0.000. Z value of HER2 expression was 2.752, and P value was 0.006. Z value was 3126, and P value was 0.020 for K-ras expression. In the study of the correlation of β2 AR expression with HER2 and K-ras, the expression of β2 AR and HER2 and the expression of β2 AR and K-ras were correlated. The difference in expressions of β2 AR was statistically significant only in groups with or without lymph node metastasis, but not in groups varied in age, tumor size, clinical stage, pathological grading or hormone level. Conclusion The high expression of β2 AR, HER2, and K-ras in breast cancer tissues is associated with the occurrence and development of breast cancer. There is also a greater risk of potential lymph node metastasis in breast cancer with high expression of β-2 AR. β-2 AR may play a role in pathogenesis of breast cancer through HER2 and K-ras.