高效液相色谱法检测水中的氟噻草胺

Determination of Flufenacet in Water by HPLC

[目的]建立高效液相色谱法检测水中的氟噻草胺。[方法]水样经乙腈提取,以乙腈-0.1%甲酸水(体积比70∶30)为流动相,Agilent TC-C_(18)(2)色谱柱分离,在225 nm检测波长下,对氟噻草胺进行定量测定。[结果]在1～100mg/L范围内,氟噻草胺的质量浓度与对应的峰面积间呈良好的线性关系;在添加水平为5、50 mg/L时,氟噻草胺在水中的平均回收率为89.4%～103.1%,相对标准偏差(RSD)分别为4.7%和1.2%。[结论]该方法操作简便、快捷,线性关系良好,准确度、精密度符合农药定量分析要求,可用于水中氟噻草胺的定量分析检测。

[Aims] An analysis method using high performance liquid chromatography(HPLC) was developed for the determination of flufenacet in water. [Methods] The water samples were extracted with acetonitrile, followed by quantitative detection by HPLC using Agilent TC-C18(2) column, and with the mixture solution of acetonitrile and 0.1%formic acid water(70∶30, by vol) as mobile phase at 225 nm. [Results] There was a good linear relationship between the target substance concentration and the peak area in the range of 1-100 mg/L. Besides, the average recoveries were89.4-103.1% at the spiked levels of 5 and 50 mg/L, and the relative standard deviations(RSD) were 4.7 and 1.2%,respectively. [Conclusions] The analysis method is simple and convenient, and the accuracy and precision meet with the requirement for pesticide quantitative analysis, which can be applied for determination of flufenacet in water.