葛根素对丙烯酰胺染毒大鼠中枢神经能量代谢影响

Effect of puerarin on energy metabolism of central nervous system in acrylamide-exposed rats

目的观察葛根素对丙烯酰胺染毒大鼠中枢神经能量代谢的影响。方法无特定病原体级成年雄性SD大鼠随机分为对照组、模型组和葛根素低、中、高剂量组,每组10只。模型组和葛根素低、中、高剂量组大鼠均予剂量为30 mg/kg体质量丙烯酰胺腹腔注射染毒,对照组大鼠腹腔注射等体积的0. 9%氯化钠溶液;染毒1 h后,3个葛根素剂量组大鼠分别予剂量为40、80和160 mg/kg体质量葛根素腹腔注射,每周3次,连续给药4周。于第4周,断头处死大鼠后分离其脑和脊髓,检测脑和脊髓线粒体中腺苷二磷酸(ADP)/腺苷三磷酸(ATP)比值、ATP合成酶活力及线粒体膜电位。结果与对照组比较,模型组和3个葛根素剂量组大鼠脑与脊髓组织线粒体中ADP/ATP比值均升高(P <0. 05),ATP合成酶活力和线粒体膜电位均下降(P <0. 05)。与模型组比较,3个葛根素剂量组大鼠脑与脊髓组织线粒体中ADP/ATP比值均下降(P <0. 05),脑组织线粒体中线粒体膜电位均升高(P <0. 05)。与模型组比较,葛根素高剂量组大鼠脑与脊髓组织ATP合成酶活力均升高(P <0. 05),葛根素中、高剂量组大鼠脊髓组织线粒体膜电位均升高(P <0. 05)。随着葛根素剂量的增加,大鼠脑和脊髓组织线粒体中ADP/ATP比值下降(P <0. 05),线粒体膜电位升高(P <0. 05)。结论葛根素可能通过调节线粒体ATP合成酶增强大鼠中枢神经线粒体的能量代谢功能,并对丙烯酰胺染毒大鼠中枢神经线粒体膜有一定的保护作用。

Objective To observe the effect of puerarin on energy metabolism of the central nervous in acrylamide-exposed rats. Methods Specific pathogen free adult male SD rats were randomly divided into control group,model group,and puerarin low-,medium-,and high-dose groups,with 10 rats in each group. Intraperitoneal injection of acrylamide was given to rats in model group,and puerarin low-,medium-,and high-dose groups( 30 mg/kg body weight). Rats in control group were intraperitoneally injected with equal volume of 0. 9% sodium chloride solution. Rats in the puerarin low-,medium-,and high-dose groups were given puerarin of 40,80 and 160 mg/kg body weight,respectively after one hour of acrylamide exposure,three times a week for continuous 4 weeks. In the 4th week,the rats were sacrificed,the brain and spinal cord were isolated,and the ratio of adenosine diphosphate( ADP)/adenosine triphosphate( ATP),ATP activity and mitochondrial membrane potential( MMP) in brain and spinal cord tissue mitochondria were detected. Results The ADP/ATP ratio increased in mitochondria of brain and spinal cord tissue in model group and the three puerarin treatment groups( P < 0. 05),meanwhile the ATP synthase activity and MMP decreased compared with control group( P < 0. 05). The ADP/ATP ratio decreased in mitochondria of brain and spinal cord tissue( P < 0. 05),while MMP increased in mitochondria of brain tissue in the three puerarin treatment groups compared with model group( P < 0. 05). The ATP synthase activity increased in mitochondria of brain and spinal cord tissue of puerarin high-dose group( P < 0. 05),and MMP increased in mitochondria of spinal cord tissue of puerarin medium-does group and puerarin high-does group when compared with the model group( P < 0. 05). The ADP/ATP ratio in mitochondria of rat brain and spinal cord decreased with the increase of puerarin doses( P < 0. 05). The MMP of rat brain and spinal cord increased with the increase of puerarin dose( P < 0. 05). Conclusion Puerarin enhances the energy metabolism function of the central nervous in rats by regulating mitochondrial ATP activity. It has certain protective effect on the mitochondrial membrane of central nervous system in rats exposed to acrylamide.