巴马小型猪股骨髁关节软骨及软骨下骨相关参数的测量

The related parameters of cartilage and subchondral bone of femoral condyle in bama minipig

目的测量成熟雌性巴马小型猪股骨内、外侧髁与滑车尺寸及软骨厚度,为可制作软骨缺损尺寸及深度提供参考。方法测量对象为10头成熟雌性巴马小型猪,使用刻度尺分别沿股骨内、外侧髁及滑车最宽处轴线测量其宽度;采用针入度测试方法测量股骨及滑车软骨厚度;MicroCT扫描股骨远端标本,通过SCANCO Medical软件分析获得股骨及滑车软骨下骨板厚度、骨密度、骨小梁厚度及骨体积分数,并三维重建骨小梁结构。结果 小型猪与人具有较为相似的软骨下骨板厚度及骨小梁厚度,成熟雌性巴马小型猪股骨内侧髁宽度为(16.4±1.2) mm,股骨外侧髁宽度为(15.11±0.95) mm,滑车内侧面宽度为(11.1±0.6) mm,滑车外侧面宽度为(9.0±0.6) mm,软骨厚度[股骨内侧髁:(1.5±0.2) mm,股骨外侧髁:(1.55±0.08) mm,滑车内侧面:(0.8±0.2) mm,滑车外侧面:(0.85±0.03) mm],软骨下骨板厚度[内侧髁:(207±95) μm,滑车:(95±36) μm],骨密度[内侧髁:(0.72±0.12) g/cm^3,滑车:(0.77±0.15) g/cm^3],骨体积分数[内侧髁:(46±7)%,滑车:(35±11)%],骨小梁厚度[内侧髁:(149±15) μm,滑车:(141±12) μm];并且通过三维重建骨小梁,发现小型猪与人在骨小梁结构及排列上具有良好的相似性。结论 成熟巴马小型猪具有较大的股骨内侧髁尺寸,可用于制作直径超过10 mm的较大软骨缺损;并且其在软骨厚度上具有一定的优势,可用于制作部分软骨、全层软骨或骨软骨缺损模型。

Objective To measure the size and thickness of cartilage in femoral condyle and femoral trochlea of mature bama minipig, thus providing a reference for the doable cartilage defect size and depth.Methods The measuring objects were 10 mature female minipigs, scale was used to measure the size of medial and lateral femoral condyle and femoral trochlea, using needle penetration test method for measuring the thickness of cartilage, microCT scanning was applied to measure the bone mineral density, bone volume fraction and trabecular thickness of subchondral bone.Results The thickness of subchondral bone plate and trabeculae were similar in human and minipig. The width of medial femoral condyle was (16.4±1.2) mm, lateral femoral condyle was (15.11±0.95) mm, the width of trochlear medial facet was (11.1±0.6) mm, the lateral facet was (9.0±0.6) mm, cartilage thickness [medial femoral condyle: (1.5±0.2) mm, lateral femoral condyle: (1.55±0.08) mm, medial trochlear facet: (0.8±0.2) mm, lateral trochlear facet: (0.85±0.03) mm], subchondral bone plate thickness [medial condyle: (207±95) μm, trochlea: (95±36) μm], bone mineral density [medial condyle: (0.72±0.12) g/cm^3, trochlea: (0.77±0.15) g/cm^3], bone volume fraction [medial condyle: (46±7) %, trochlea: (35±11) %], trabecular thickness [medial condyle: (149±15) μm, trochlea: (141±12) μm];The arrangement and structure of trabeculae in minipig were consistent with that of human.Conclusion Larger cartilage defect with more than 10 mm in diameter can be made in bama minipig due to its advantage of wider medial femoral condyle. Partial full-thickness cartilage defect can also be made owing to its thicker cartilage.