日本矶海绵共生真菌分离及其代谢产物抑菌活性研究

Isolation and identification of symbiotic fungi from marine sponge Reniera japonica and the antimicrobial evaluation of its crude extract

以日本矶海绵作为试材,采用3种不同培养基利用涂布法以及划线法对其共生真菌进行分离。共分离到33株真菌,通过菌落形态、显微形态、生长规律观察并结合rDNA ITS序列分析,确定各菌株的种属,发现曲霉属和青霉属真菌为优势菌,其中M3是杂色曲霉。以致病菌大肠埃希氏菌(Escherichia coli)标准菌株为测试菌,采用96孔板法研究矶海绵共生真菌代谢产物的抑菌活性。结果表明,有7株共生真菌的次生代谢产物具有一定的抑菌活性,其中M3代谢产物对大肠杆菌的抑制作用最强,抑制率达(56.66±2.28)%,其次是M1,抑制率为(51.23±6.24)%,二者均高于100mmol/L青霉素。

Thirty-three fungal strains were isolated from the healthy tissue of marine sponge by three different media.They were identified by morphology characteristics analysis and rDNA ITS sequence analysis.The species of each strain was determined,and the dominant fungi of Aspergillus and Penicillium were found.Among them,M3 was Aspergillus versicolor.The antimicrobial activity of the metabolites of the symbiotic fungi of marine sponge was studied by 96-well plate method with the standard strain of Escherichia coli.The results showed that the secondary metabolites of seven strains of symbiotic fungi had certain antimicrobial activity.The inhibition rate of M3 on Escherichia coli was(56.66±2.28)%,followed by M1,with the inhibition rate was(51.23±6.24)%.Both of them of higher than 100 mmol/L penicillin.