泛素结合酶UBE2T在结直肠癌中的表达及其生物学作用

The expression pattern and biological role of ubiquitin-binding enzyme UBE2T in colorectal cancer

目的探索泛素结合酶UBE2T在结直肠癌的表达及其生物学作用。方法运用免疫组化法检测78对结直肠癌和对应癌旁正常组织中UBE2T的蛋白表达水平。脂质体转染法将UBE2T-siRNA及对照NC-siRNA转染结直肠癌细胞SW480后,采用细胞计数试剂盒(CCK-8)及平板克隆实验分析增殖情况;流式细胞仪检测细胞周期情况;Western blotting检测细胞中UBE2T、cyclin D1、cyclin E等蛋白表达情况。结果与正常结直肠组织相比,结直肠癌组织中UBE2T表达水平增高(P<0.001)。癌组织中UBE2T异常高表达与不良病理指标相关:肿瘤大小(P=0.014)、组织分化程度(P=0.005)、浸润深度(P=0.033)和TNM分期(P=0.014)。CCK-8增殖实验和平板克隆形成实验都提示沉默UBE2T表达能显著抑制结直肠癌细胞的增殖。沉默UBE2T抑制周期蛋白cyclin D1和cyclin E的表达,促进结直肠癌细胞发生G1/S期细胞周期阻滞。结论 UBE2T在结直肠癌组织中异常高表达,并与不良病理指标相关。沉默UBE2T通过促进G1/S期细胞周期阻滞抑制结直肠癌细胞的增殖。

Objective To investigate the expression pattern and biological role of ubiquitin-binding enzyme UBE2 T in colorectal cancer.Methods Immunohistochemistry was used to detect the protein expression of UBE2 T in 78 pair specimens of colorectal cancer and corresponding normal tissues.After UBE2T-siRNA and control NC-siRNA were transfected into colorectal cancer cell SW480 by lipofectamine 2000,proliferation was evaluated by cell counting kit(CCK-8) and plate clone assay.Cell cycle was detected by flow cytometry.The protein expression of UBE2 T,cyclin D1 and cyclin E were detected by Western blotting.Results Compared with normal colorectal tissue,the expression of UBE2 T in colorectal cancer was significantly increased(P < 0.001).The abnormally high expression of UBE2 T in cancerous tissues was associated with poor pathological features:tumor size(P = 0.014),histological grade(P = 0.005),depth of invasion(P = 0.033) and TNM stage(P = 0.014).Both CCK-8 proliferation assay and plate clone formation assay suggested that silencing UBE2 T expression could significantly inhibit the proliferation of colorectal cancer cells.Silencing UBE2 T inhibits the expression of cyclin D1 and cyclin E,and promotes G1/S cell cycle arrest in colorectal cancer cells.Conclusion UBE2 T is highly expressed in colorectal cancer tissues and is associated with poor pathological parameters.Silencing UBE2 T inhibits the proliferation of colorectal cancer cells by promoting G1/S cell cycle arrest.