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大黄对新生大鼠支气管肺发育不良的保护作用 被引量:4

Protective effect of Rhubarb on newborn rats with bronchopulmonary dysplasia
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摘要 目的探讨大黄对支气管肺发育不良(BPD)的影响及其可能的作用机制。方法将出生4 d的SD大鼠按随机数字表法随机分为4组(每组16只):空气+9 g/L盐水组、空气+大黄组、高氧+9 g/L盐水组、高氧+大黄组。前2组暴露于空气中,后2组暴露于600 mL/L氧中。大黄干预组(空气+大黄组、高氧+大黄组)大鼠每天予大黄600 mg/kg灌胃,对照组(空气+9 g/L盐水组、高氧+9 g/L盐水组)大鼠给予等量9 g/L盐水灌胃。各组新生鼠于出生后14、21 d按随机数字表法随机抽取8只,麻醉后处死并取肺组织。苏木精-伊红(HE)染色检测肺组织形态学改变,原位末端标记法(TUNEL)检测肺组织细胞凋亡,Western blot检测Fas蛋白表达,分光光度计检测Caspase-8和Caspase-3酶活性。结果与空气+9 g/L盐水组比较,高氧+9 g/L盐水组于大鼠出生后14、21 d时均可见明显的肺组织病理改变(肺泡数目减少,体积增大,肺结构简单化),肺组织细胞凋亡指数(39.91±1.91比10.11±1.64,48.80±4.51比12.90±3.18)、Fas蛋白表达量(0.47±0.02比0.21±0.01,0.55±0.02比0.22±0.01)、Caspase-8(52.59±1.23比40.74±1.08,60.20±3.48比40.39±2.47)和Caspase-3酶活性(57.17±1.88比42.00±1.19,64.57±2.79比41.54±1.27)均显著增加,差异均有统计学意义(均P<0.001)。高氧+大黄组与相应时间点的高氧+9 g/L盐水组比较,肺组织病理损伤明显改善,肺组织凋亡细胞减少(26.49±2.65比22.97±3.66),Fas蛋白表达量降低(0.27±0.03比0.31±0.01),Caspase-8(32.70±2.69比30.66±4.48)和Caspase-3(44.94±1.60比44.59±1.66)酶活性均明显降低,差异均有统计学意义(均P<0.001)。结论大黄可通过减少新生大鼠肺组织细胞凋亡减轻BPD的高氧肺损伤,其机制可能涉及Fas凋亡途径。 Objective To investigate the effect of Rhubarb on bronchopulmonary dysplasia(BPD) and its potential mechanism. Methods Neonatal Sprague-Dawley rats (postnatal day 4) were exposed to 600 mL/L O2 to induce BPD.The experimental rats were randomly divided into 4 groups with 16 in each group: air+ saline group, air+ Rhubarb(600 mg/kg)group, and hyperoxia+ saline group, and hyperoxia+ Rhubarb group.The rats were sacrificed and lung tissues were obtained on day 14 and 21 after birth.Hematoxylin-easin staining was used to detect the pathomorphology of the lungs.Apoptosis of the lung tissue was detected by means of TdT-mediated dUTP nick-end labeling(TUNEL). The expression of Fas was detected by adopting Western blot.The activity of Caspase-8 and Caspase-3 was detected by using spectrophotometer. Results The lung structure of rats was markedly abnormal (decreased, enlarged and simplified alveoli) after being exposed to hyperoxia at any time point.The apoptosis indexes (39.91±1.91 vs.10.11±1.64, 48.80±4.51 vs.12.90±3.18), the expression levels of Fas (0.47±0.02 vs.0.21±0.01, 0.55±0.02 vs.0.22±0.01) and the activities of Caspase-8 (52.59±1.23 vs.40.74±1.08, 60.20±3.48 vs.40.39±2.47) and Caspsase 3 (57.17±1.88 vs.42.00±1.19, 64.57±2.79 vs.41.54±1.27) were all increased in the hyperoxia+ saline group compared with those in the air+ saline group on day 14 and 21, and the differences were statistically significant(all P<0.001). However, compared with the hyperoxia+ saline group, Rhubarb administration dramatically decreased pulmonary apoptosis index (26.49±2.65 vs.22.97±3.66), Fas protein level (0.27±0.03 vs.0.31±0.01) and the activity of Caspase-8 (32.70±2.69 vs.30.66±4.48) and Caspase-3 (44.94±1.60 vs.44.59±1.66), and the differences were statistically significant(all P<0.001). Meanwhile, lung damage after hyperoxia was significantly attenuated in the hyperoxia+ Rhubarb group. Conclusion Rhubarb can reduce the hyperoxic lung injury of BPD by reducing the apoptosis of newborn rat lung tissue cells, and the mechanism may involve the apoptosis pathway of Fas.
作者 尹玲玲 唐丽君 黄为民 Yin Lingling;Tang Lijun;Huang Weimin(Depdrtment of Neonatology,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China)
出处 《中华实用儿科临床杂志》 CSCD 北大核心 2019年第1期60-63,共4页 Chinese Journal of Applied Clinical Pediatrics
关键词 支气管肺发育不良 大黄 凋亡 Bronchopulmonary dysplasia Rhubarb Apoptosis
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