~1HNMR指纹图谱识别不同产地的大花红景天

Identification of the different origins of Rhodiola crenulata by ~1HNMR fingerprint

目的采用~1HNMR指纹图谱识别不同产地的大花红景天。方法采用傅立叶变换NMR仪测定4个不同产地大花红景天的特征性全成分信息,得到~1HNMR指纹图谱,将图谱进行处理转化为数据矩阵,结合相似度评价和化学模式识别方法中的主成分分析(PCA)、聚类分析法(HCA)进行化学模式识别分析。结果采用~1HNMR指纹图谱结合化学模式识别法能够有效鉴别4个不同产地的大花红景天。结论基于~1HNMR指纹图谱结合化学模式识别方法可作为一种分类及鉴别不同产地的大花红景天的方法。

OBJECTIVE To identify the different origins of Rholdiola crenulata by ^1HNMR fingerprints.METHODS ^1HNMR spectra of R.crenulata from four different origins was obtained using the Fourier transform NMR spectrometer to get the characteristic full composition information of samples,and converted into a data matrix.Combined with the similarity evaluation,the principal component analysis(PCA)and the cluster analysis (HCA)in the chemical pattern recognition method were used for chemical pattern recognition analysis.RESULTS ^1 HNMR fingerprint combined with chemical pattern recognition method can effectively identify R.crenulata from four different origins.CONCLUSION The method based on ^1 HNMR fingerprint pattern combined with chemical pattern recognition can be used to classify and identify R.crenulata from different origins.