摘要
为研究维氏气单胞菌ndk基因的功能,利用自杀性质粒pRE112通过同源重组的方法缺失ndk基因,然后对野生株和缺失株的生长能力、运动性、生物被膜形成能力及LD50等进行差异分析。经过PCR以及荧光定量PCR表达水平证明ndk基因缺失成功;生物学特性研究显示,缺失株的生长能力和泳动性均下降;生物被膜形成能力与野生株相比差异极显著(P<0.001);与野生株相比,LD50升高了5.5倍,且差异极显著(P<0.001)。由此可以推测,ndk基因与维氏气单胞菌的毒力及生物被膜形成密切相关。本研究结果为维氏气单胞菌致病机制的研究奠定了基础。
In order to study the function of ndk gene in Aeromonas veronii, the ndk gene mutant was constructed using suicide plasmid p RE112 by homologous recombination,then the differences between wild and mutant strains in growth ability,motility,biofilm formation and LD50 were analyzed.The results of PCR and expression of fluorescence quantitative PCR showed that ndk gene mutant was confirmed.The biological characteristics showed that the decrease of the growth ability and the migratory ability on the deleted strains.The formation ability of biofilm as well as the increased 5.5-fold of LD50 in the mutant strains were significantly different from the wild strain TH0426(P<0.001).The studies suggested that the ndk gene of A.veronii was closely related to the virulence and biofilm formation.The results laid the foundation for the study on pathogenesis of A.veronii.
作者
宋明芳
张海鹏
张冬星
张蕾
康元环
孙武文
单晓枫
钱爱东
SONG Ming-fang;ZHANG Hai-peng;ZHANG Dong-xing;ZHANG Lei;KANG Yuan-huan;SUN Wu-wen;SHAN Xiao-feng;QIAN Ai-dong(College of Animal Science and Technology,Jilin Agricultural University,Chongchun 130118,China)
出处
《中国兽医科学》
CAS
CSCD
北大核心
2019年第2期206-212,共7页
Chinese Veterinary Science
基金
国家自然科学基金项目(31201927)
吉林省重点科技攻关项目(20150204065NY)
吉林省科技厅自然科学基金学科布局项目(20170101016JC)
吉林省教育厅项目(JJKH20180694KJ)
关键词
维氏气单胞菌
ndk基因
自杀性质粒
生物学特性
Aeromonas veronii
nucleoside diphosphate kinase gene
suicide plasmid
biological characterizations