ADIPOR1基因多态性与冠状动脉多支血管严重病变合并心功能不全的相关性

Association of ADIPOR1 gene polymorphism with severe multi-vessel coronary disease and cardiac insufficiency

目的对比研究冠状动脉多支血管严重病变合并心功能不全的患者与正常人群ADlPORl基因多态性的差异。方法连续选取2017年2月至2018年4月于我病房住院行冠状动脉造影提示多支血管严重病变且合并心功能不全的62例患者作为冠心病组,另选取同期住院行冠状动脉造影的51例非冠心病非糖尿病患者为对照组,分析ADIPORl基因的12个单核苷酸多态性(single nucleotide polymorphism,sNPs)与冠心病、冠心病合并2型糖尿病的关系。结果①rs7529354、rs7514221、rs2275737、rs12045862的风险等位基因在冠心病组出现的频率更高(P均<0.05)。②进一步将冠心病组患者分为单纯冠心病组(单纯CAD组)、冠心病合并2型糖尿病组(CAD+T2D组),将两组患者的基因型分布分别与对照组进行比较发现,rs7529354的G等位基因携带者(GG+AG)患冠心病的风险是AA基因型携带者的9.7倍(OR=9.7,95%CI 1.0-91.2,P=0.047);rs7514221的A等位基因携带者(AA+AG)患冠心病合并2型糖尿病的风险是GG基因型携带者的8.2倍(OR=8.2,95%CI 1.6-42.0,P=O.012)。rs2275737的G等位基因携带者(GG+TG)患冠心病的风险是TT基因型携带者的3.7倍(OR=3.7,95%CI 1.0-13.2,P=0.046),其患冠心病合并2型糖尿病的风险是TT基因型携带者的4.9倍(OR=4.9,95%CI 1.2-20.1,P=0.029);rs12045862的A等位基因携带者(AA+AG)患冠心病合并2型糖尿病的风险是GG基因型携带者的3.5倍(017=3.5,95%CI 1.2-10.1,P=0.023)。rs1342387的G等位基因携带者(GG+AG)发生冠心病的风险是AA基因型携带者的2.6倍(OR=2.6,95%CI 1.1-6.3,P=0.035)。③单倍型分析中,由rs2275737(T>G)、rs7514221(G>A)及rs7529354(A>G)构成的保护性单倍型AGT与CAD合并T2D的易感性呈负相关(OR=0.09,95%CI 0.02-0.48,P=0.006)。结论ADIPOR1基因rs7529354、rs7514221、rs2275737、rs12045862、rs1342387多态性可能与汉族人群冠心病的易感性相关。

Objective To compare the polymorphism of ADIPOR1 gene in patients with severe multivessel coronary artery disease complicated with cardiac insufficiency and normal controls.Methods From February 2017 to April 2018,62 patients with coronary artery disease (CAD)who underwent coronary angiography in our ward were selected as CAD group."In addition,51 non-CAD/T2DM patients who were hospitalized at the same time were selected as control group.Then to analyze the relationship between 12 single nucleotide polymorphisms (SNPs)of ADIPOR1 gene and CAD complicated with type 2 diabetes mellitus (T2DM).Results (1)Compared with normal control group,the risk allele of rs7529354,rs7514221,rs2275737 as well as rs12045862 was more frequent in CAD group (P<0.05).(2)The patients in CAD group were further divided into simple CAD group (CAD group )and CAD complicated with T2DM group (CAD+T2DM group ).The genotype distribution of the two groups was compared with control group respectively.It shows that the G allele carriers of rs7529354 are 9.7 times more likely to develop CAD in(GG+AG)than those with AA genotype (OR= 9.7,95%CI 1.0-91.2,P=0.047).The risk of CAD associated with T2DM in rs7514221-A allele carrier(AA+AG) was 8.2 times higher than that in GG genotype carrier (0R=8.2,95%CI 1.6-42.0,P=0.012).rs2275737-G allele carriers were 3.7 times more likely to develop CAD than TT genotype carriers (0R=3.7,95%CI 1.0-13.2,P= 0.046).The risk of CAD associated with T2DM was 4.9 times as high as that of TT genotype carriers (0R=4.9, 95%C1 1.2-20.1,P=0.029).The risk of CAD associated with T2DM in rs12045862-A allele carrier (AA+AG) was 3.5 times higher than that in GG genotype carriers(OR=3.5,95%CI 1.2-10.1,P=0.023).rs1342387-G allele carriers were 2.6 times more likely to develop CAD than those with AA genotype carriers (OR=2.6,95%CI 1.1- 6.3,P=0.035).(3)In haplotype analysis,the protective haplotype AGT composed of rs2275737 (T>G), rs7514221 (G>A)and rs7529354 (A>G)was negatively correlated with the susceptibility of CAD with T2DM (OR=0.09,95%CI 0.02-0.48,P=0.006).Conclusion The polymorphism of rs7529354,rs7514221,rs2275737, rs12045862,rs1342387 in ADIPORI gene may be associated with the susceptibility to coronary artery disease in Chinese Hart nationality.