EB病毒抑制小肠上皮细胞增殖的ERK1/2途径分析

Effect of EB virus on growth of intestinal epithelial cells via ERK1/2 pathway

目的分析EB病毒(EB virus,EBV)抑制小肠上皮细胞增殖的机理,初步探讨EBV导致腹泻的病理机制。方法应用EBV作用于小肠上皮细胞,作用后0、12、24、36和48 h,采用MTT法检测细胞的增殖情况,Western blot法检测细胞MAPK(磷酸化的ERK1/2、JNK、p38)通路的表达情况。结果经EBV作用36及48 h的小肠上皮细胞存活率较24及12 h显著降低,并显著低于0 h(P <0. 05);磷酸化ERK1/2的表达在36和48 h最低,显著低于其他3个时间点(P <0. 05)。结论 EBV可抑制小肠上皮细胞的增殖,且通过抑制ERK1/2磷酸化途径实现。本实验为进一步研究EBV导致腹泻的发病机理提供了参考。

Objective To analyze the mechanism of EB virus(EBV)in inhibiting the proliferation of intestinal epithelial cells and causing diarrhea.Methods Intestinal epithelial cells were inoculated with EBV for 0,12,24,36 and 48 h,and determined for proliferation by MTT assay.The expression of MAPK(phosphorylated ERK1/2,phosphorylated JNK and phosphorylated p38) pathway was determined by Western blot.Results The survival rates of intestinal epithelial cells 36 and 48 h were significantly lower than those 12 and 24 h after inoculation with EBV as well as that before inoculation(0 h,P<0.05).However,the expression levels of phosphorylated ERK1/2(p-ERK1/2) 36 and 48 h were significantly lower than those at the other three time points after inoculation(P<0.05).Conclusion EBV inhibited the proliferation of intestinal epithelial cells via ERK1/2 pathway.It laid a foundation of further study on the pathological mechanism of diarrhea caused by EBV.