阳和汤含药血清对乳腺癌干细胞增殖的影响

Effect of Yanghe decoction serum on the proliferation of breast cancer stem cells

目的观察阳和汤含药血清对乳腺癌干细胞HMLER90hi增殖的影响,探讨其作用机制。方法将20只雌性SD大鼠按随机数字表法分为阳和汤低、中、高剂量组和对照组,制备阳和汤含药血清和空白血清。采用MTT法检测各组在24、48、72 h对HMLER90hi细胞增殖的影响;实时荧光定量PCR检测各组细胞EphA4、NF-κB p50 mRNA表达水平。结果与对照组比较,阳和汤低、中、高剂量组细胞24 h[(0.818±0.061)、(0.706±0.073)、(0.587±0.052)比(0.928±0.075)]、48 h[(0.760±0.047)、(0.638±0.056)、(0.510±0.059)比(0.973±0.095)]、72 h[(0.672±0.102)、(0.508±0.092)、(0.448±0.048)比(1.023±0.099)]增殖活性均降低(P<0.05或P<0.01)。药物干预24 h后,与对照组比较,阳和汤低、中、高剂量组EphA4 mRNA[(0.54±0.07)、(0.54±0.07)、(0.33±0.04)比(0.68±0.09)]、NF-κB p50 mRNA[(0.69±0.10)、(0.54±0.08)、(0.41±0.06)比(0.85±0.13)]表达降低(P<0.05或P<0.01)。结论阳和汤可抑制乳腺癌干细胞HMLER90hi增殖,其作用机制可能与抑制单核巨噬细胞近分泌通路的传导有关。

Objective To investigate the effect of Yanghe decoction serum on the proliferation of breast cancer stem cells HMLER90hi and its mechanism.Methods Twenty female Sprague-Dawley rats were randomly divided into low,medium and high dose groups containing drug serum and control group,in order to prepare the Yanghe decoction serum and blank serum. After 24 hours of drug intervention,the effects of each group on the proliferation of HMLER90hi cells at 24 h,48 h,and 72 h were detected by MTT assay. The expression of EphA4 and p50 mRNA in each group were detected by real-time fluorescence quantitative PCR.Results Compared with the blank control group,the cell proliferation activity of each dose group of Yanghe decoction significantly decreased at 24 h (0.818 ± 0.061,0.706 ± 0.073,0.587 ± 0.052 vs. 0.928 ± 0.075),48h (0.760 ± 0.047,0.638 ± 0.056,0.510 ± 0.059 vs. 0.973 ± 0.095),and 72 h (0.672 ± 0.102,0.508 ± 0.092,0.448 ± 0.048 vs. 1.023 ± 0.099) (P<0.05 or P<0.01),respectively. After 24 h of drug intervention,compared with the control group,the expression of EphA4 mRNA (0.54 ± 0.07,0.54 ± 0.07,0.33 ± 0.04 vs. 0.68 ± 0.09) significantly decreased,and p50 mRNA (0.69 ± 0.10,0.54 ± 0.08,0.41 ± 0.06 vs. 0.85 ± 0.13) significantly decreased in each dose group of Yanghe decoction (P<0.05 or P<0.01).Conclusions The Yanghe decoction can inhibit the proliferation of breast cancer stem cell HMLER90hi,and its mechanism may be related to its inhibition of the conduction of the juxtacrine pathway of monocyte macrophage.