头花蓼提取物中具抗淋球菌作用的有效部位研究

Study on effective sites of anti-gonococcus in Polygonum capitatum extract

目的分析头花蓼提取物中具有抗淋球菌活性有效部位的成分。方法采用药敏纸片法检测头花蓼提取物的不同部位对淋球菌的抑菌活性。并采用UPLC-TOF-MS对头花蓼提取物中具有抗淋球菌作用的有效部位进行鉴别。结果头花蓼醇提取物经特定的大孔树脂洗脱所获得的洗脱物中,35%甲醇洗脱物(部位F3)中含有可水解鞣质(三没食子酰葡萄糖),该部位具有良好的抗淋球菌作用。F2、F4～6有微弱的抑菌作用,而其他部位无抑菌作用。结论头花蓼提取物的35%甲醇洗脱物具有良好的抗淋球菌作用,其主要成分为三没食子酰葡萄糖,可能为头花蓼抗淋球菌物质基础。

Objective To analyze the components of active anti-gonococcal fractions in the extracts of Polygonum capitatum.Methods Drug sensitive paper method and Neisseria gonorrhoeae culture method were used to detect the antibacterial activity of different parts of the flower bud extract against Neisseria gonorrhoeae.UPLC-TOF-MS was used to identify the effective parts of the flower bud extract with anti-gonococcal effect.Results As a result,in the eluate obtained by extracting with a specific macroporous resin,the hydrolysable tannin was contained in the methanol(35%) eluate(fraction F3),such as trigallocyl glucose.This fraction had a good anti-gonococcal effect.The fractions of F2 and F4-6 had a weak antibacterial effect,while other fractions have no antibacterial effect.Conclusion This study provides a basis for the use of trigalactosyl glucose in the preparation of anti-gonococcal drugs,and lays a foundation for the development of a clinical drug of P.capitatum extracts against Neisseria gonorrhoeae.