剑叶耳草中的1个新咖啡酸苷化学成分及其抗肿瘤活性

A new caffeoyl glucoside from Hedyotis caudatifolia with antitumor activity

目的研究剑叶耳草Hedyotiscaudatifolia的化学成分。方法采用硅胶、聚酰胺、SephadexLH-20凝胶等柱色谱技术进行分离纯化,通过化合物的核磁共振、质谱等光谱数据和理化性质鉴定其结构。结果从剑叶耳草的甲醇提取物中分离得到10个化合物,其结构分别鉴定为剑叶耳草苷A(1)、β-谷甾醇(2)、5α-豆甾烷-3,6-二酮(3)、7-羟基-6-甲氧基香豆素(4)、齐墩果酸(5)、乌苏酸(6)、坡模酸(7)、3α,19α-二羟基-12-烯-24, 28-乌苏酸(8)、2α,3β,24-三羟基-12-烯-28-齐墩果酸(9)、2α,3α-二羟基-12-烯-28-齐墩果酸(10)。结论化合物1为新的咖啡酸葡萄糖苷,化合物7～10均为首次从该植物中分离得到。化合物1具有一定的抑制肿瘤细胞生长活性。

Objective To study the chemical constituents of Hedyotis caudatifolia. Methods These compounds were isolated from H. caudatifolia and purified by repeated silica gel, polyamine, Sephadex LH-20 column chromatography and other methods. Their structures were identified by NMR, MS, and physico-chemical properties. Results Ten compounds were isolated and elucidated as hedycaffeoylglucoside A(1), β-sitosterol(2), 5α-stigmastane-3,6-dione(3), 7-hydroxy-6-methoxycoumarin(4), oleanolic acid(5), ursolic acid(6), pomolic acid(7), 3α,19α-dihydroxyurs-12-en-24,28-dioic acid(8), 2α,3β,24-trihydroxyolean-12-en-28-oic acid(9), and 2α,3α-dihydroxyolean-12-en-28-oic acid(10), respectively. Conclusion Compound 1 is a new caffeoyl glucoside and compounds 7-10 are isolated from this plant for the first time. Compound 1 exhibits cytotoxicity against four tumor cells.