枸杞玻璃化组培苗恢复技术

Restoration of Vitrified Test-tube Plantlets of Lycium barbarum

以枸杞不同程度玻璃化组培苗为试材,采用组织培养法,研究了不同基本培养基、外源添加物、添加聚丙烯酰胺、改变培养条件及玻璃化苗脱水处理在玻璃化组培苗恢复中的作用,探索出最佳的枸杞玻璃化组培苗恢复方法。结果表明:1/2MS+6-BA 0.5mg·L^(-1)+活性炭0.5g·L^(-1)为枸杞轻度玻璃化苗恢复最佳培养基,适宜枸杞中度玻璃化苗恢复培养基为1/4MS+6-BA 0.5mg·L^(-1)+活性炭1.0g·L^(-1)。当添加聚丙烯酰胺8、16g·L^(-1),枸杞轻度、中度玻璃化苗恢复率均达到最高,聚丙烯酰胺对枸杞玻璃化的组培苗有明显的逆转效果,逆转后的组培苗生长良好。经过脱水处理后,玻璃化苗明显得到恢复。脱水2d轻度玻璃化苗的恢复率可达77.61%。随着脱水的天数增加,枸杞中度玻璃化苗恢复率逐渐提高,当脱水天数达3d时,恢复率最高达71.24%。培养期间增强封口膜的透气性和光照强度能有效提高枸杞玻璃化苗恢复率。

The effects of different basic medium,exogenous additives,adding polyacrylamide,different culture conditions and shoots of dehydration on vitrification shoots recovery by different vitrified seedlings of Lycium barbarum as explants for tissue culture were studied.The main purpose of the study was explore the measures of which make the vitrification shoots recovery.The results showed that 1/2MS+6-BA 0.5mg·L^-1+activated carbon 0.5g·L^-1 was the best medium for the mild vitreous seedlings recovery of Lycium barbarum.It was suitable for the moderate vitrified seedlings recovery of Lycium barbarum 1/4 MS+6-BA 0.5 mg·L^-1+activated carbon 1.0g·L^-1.When polyacrylamide 8g·L^-1 and 16g·L^-1 were added,the recovery rate of mild vitreous and moderate vitrified seedlings of Lycium barbarum was the highest.It not only could significantly inhibit the generation of vitrfication of Lycium barbarum,but also had significant reversal effect with vitrified tissue-culturing plantlets,which grew well after reverse.After dehydration treatment,the vitrified seedlings were obviously recovered.The recovery rate of light vitrified seedlings of dehydrated 2days could reach 77.61%.With the increase of the number of days of dehydration,the recovery rate of medium vitrified seedlings of Lycium barbarum was gradually increased.When the number of dehydration days reached 3days,the highest recovery rate could reach 71.24%.Enhancing membrane permeability and light intensity could also raise the recovery rate of vitrified seedlings.