诺丽果渣成分分析及抗氧化性研究

Primary functional components and antioxidant activity of different solvents extracts of noni pomace

目的分析诺丽果渣成分及抗氧化活性,为诺丽加工副产物诺丽果渣的开发利用提供理论依据。方法通过分光光度法测定诺丽果渣中的总黄酮、黄酮甙、多糖、皂甙和果胶,采用过氧化氢体系、超氧阴离子体系、羟基自由基体系和Oyaizu法,分别对诺丽果渣水提取物(waterextract,WE)、石油醚提取物(petroleum ether extract, PEE)、乙酸乙酯提取物(ethyl acetate extract, EAE)、氯仿提取物(chloroform extract, CE)和正丁醇提取物(n-butanol extract, BE)的抗氧化活性进行测定和比较。结果诺丽果渣含有总黄酮、黄酮甙、多糖、皂甙和果胶等功能成分,诺丽果渣的5种提取物在过氧化氢体系中,对H_2O_2的清除能力强弱顺序依次为VC>CE>EAE>BE>PEE>WE;在超氧阴离子体系中,对O_2^-·抗氧化活性强弱顺序依次为VC>EAE>WE>BE>PEE>CE,在羟基自由基氧化体系中,对?OH抗氧化活性强弱,依次为VC>BE>WE>EAE>PEE>CE;还原能力强弱,依次为EAE>BE>CE>WE>PEE。结论诺丽果渣5种提取物都有抗氧化性,随浓度增大抗氧化能力增强,在不同的自由基体系中,自由基清除能力不同, EAE抗氧化活性较好。

Objective To analyze the composition and antioxidant activity of noni pomace,and provide a theoretical basis for the development and utilization of noni pomace which is a by-product of noni processing.Methods The total flavonoids,flavonoids glycosides,polysaccharides,saponins and pectin were determined and compared by spectrophotometry.The antioxidant activities of water extract(WE),petroleum ether extracts(PEE),ethyl acetate extract(EAE),chloroform extract,(CE) and n-butyl alcohol extract(BE) of noni pomace were determined and compared by hydrogen peroxide system,superoxide radical system,hydroxyl radical system and Oyaizu,respectively.Results The results showed that noni pomace had functional components including flavonoids,flavones glycosides,polysaccharide,saponins and pectin.In the hydrogen peroxide system,the order of the antioxidant activity of extracts was VC>CE>EAE>BE>PEE>WE.In superoxide radical system,it was VC>EAE>WE>BE>PEE>CE.In hydroxyl radical system,it was VC>BE>WE>EAE>PEE>CE.The order of the reduce ability was EAE>BE>CE>WE>PEE.Conclusion The 5 extracts have antioxidant activity,and the ability of antioxidant activity of these extracts are increased with their concentration increasing.In the different free radical system,the activity of free radical scavenging are different,and EAE has better antioxidant activity.