制备Fe_3O_4纳米酶用于检测食品中四环素类抗生素的含量

Preparation of Fe_3O_4 nanozyme for detection of tetracycline antibiotics in foods

目的基于Fe_3O_4磁性纳米粒子建立一种新颖的显色法检测食品中四环素类抗生素(tetracyclines,TCs)的含量。方法利用水热法合成Fe_3O_4纳米酶,通过红外光谱、X-射线衍射、扫描电镜、透射电镜对Fe_3O_4纳米酶进行表征。将样品进行前处理后与材料反应后采用紫外分光光度计于451nm检测四环素类抗生素含量。结果在0.2～2.0μg/mL的浓度范围内4种四环素类抗生素的线性关系良好(r>0.99)。3水平加标回收实验的回收率为89.9%～93.1%,相对标准偏差为4.39%～6.85%,满足实验要求。结论该方法快速、准确,可用于食品中四环素类抗生素的含量测定。

Objective To establish a novel colorimetric method based on Fe3O4 magnetic nanoparticles for the detection of tetracycline antibiotics in foods.Methods Fe3O4 nano-enzyme was synthesized by hydrothermal method.Fe3O4 nano-enzyme was characterized by infrared spectroscopy,X-ray diffraction,scanning electron microscopy and transmission electron microscopy.After the sample was pretreated and reacted with the material,the tetracycline antibiotics content was detected at 451 nm using an ultraviolet spectrophotometer.Results The linear relationships of four tetracycline antibiotics were good in the concentration range of 0.2-2.0 μg/mL(r>0.99).The recoveries of the 3 spiked recovery experiments were 89.9%-93.1%,and the relative standard deviations were 4.39%-6.85%,meeting the experimental requirements.Conclusion This method is rapid and accurate,which can be used for the detection of tetracycline antibiotics in food.