超高效液相色谱-串联质谱法测定禽蛋中苏丹红的残留量

Determination of Sudan red residues in eggs by ultra performance liquid chromatography-tandem mass spectrometry

目的建立禽蛋中苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ染料残留的液相色谱-串联质谱的检测方法。方法禽蛋样品中的苏丹红经溶解、冷冻离心及过滤提取纯化,经ZorbaxSB-C_(18)(2.1 mm×50 mm, 3.5μm)色谱柱,以乙腈和0.2%的甲酸水溶液为流动相进行等度洗脱,温度为35℃,流速为0.2mL/min,外标法定量。结果在1～50μg/kg的浓度范围内,线性关系良好(r>0.99),样品中的检出限0.5μg/kg,分析时间仅为5 min,加标回收率为69.5%～108.2%,相对标准偏差为1.6%～17.5%。结论该方法准确、快速、灵敏度高,适用于禽蛋中苏丹红染料的测定。

Objective To establish a method for determination of Sudan red Ⅰ,Ⅱ,Ⅲ and Ⅳ dye residues in poultry eggs by liquid chromatography-tandem mass spectrometry.Methods Sudan red was extracted and purified by dissolution,freezing centrifugation and filtration,then the samples were separated with a ZorbaxSB-C18(2.1 mm×50 mm,3.5 μm) column at the flow rate of 0.2 mL/min by isocratic elution using acetonitrile and 0.2% formic acid aqueous solution,the temperarure was maintained at 35 ℃,and quantified by external standard method.Result In the concentration range of 1-50 μg/kg,the linear relationship was good(r>0.99),the detection limit in the sample was 0.5 μg/kg,and the analysis time was only 5 min,and the standard recoveries were 69.5%-108.2%,the relative standard deviation were 1.6%-17.5%.Conclusion The method is accurate,rapid and sensitive,which is suitable for the determination of Sudan red dye in poultry eggs.