多波长RP-HPLC法同时测定盆炎净胶囊中绿原酸和芍药苷的含量

Simultaneous determination of chlorogenic acid and paeoniflorin in Penyanjing Capsules by multiple wavelengths RP-HPLC

目的:为有效控制盆炎净胶囊的质量,建立多波长RP-HPLC法同时测定盆炎净胶囊中绿原酸和芍药苷含量的分析方法。方法:仪器为安捷伦1260型高效液相色谱仪,色谱柱为:Kromasil 100-5 C18柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.1%磷酸溶液(12∶88)溶液,绿原酸、芍药苷的检测波长分别为327 nm、230 nm,流速1.0 mL·min^(-1),柱温35℃。结果:在上述色谱条件下,绿原酸、芍药苷分别在0.213 9～1.283 4μg、0.192 9～1.157 4μg范围内,其进样质量与峰面积呈良好的线性关系。回归方程:绿原酸Y=273 3.7X+154.34(r=0.999 2);芍药苷Y=1 306.8X+23.223(r=0.999 6)。绿原酸、芍药苷的平均回收率分别为99.51%、99.62%,RSD分别为0.8%、0.72%。测定样品6批,绿原酸、芍药苷的平均含量分别为0.94 mg·g^(-1)、0.92 mg·g^(-1)。结论:本方法操作简便、准确,灵敏度高、重复性好,可用于盆炎净胶囊中绿原酸和芍药苷的同时测定,为其质量控制提供借鉴。

Objective:To establish a muhiple-wavelength RP-HPLC method for the simultaneous determination of chlorogenie acid and paeoniflorin in Penyanjing capsules in order that the quality of Penyanjing capsules can be controlled effectively.Methods:Agilent 1260 high-performance liquid chromatograph was used.The chromatographic separation was performed on a Kromasil 100-5 C18 column (4.6 mm ×250 ram,5 μm)under the elution of acetonitrile -0.1% phosphoric acid aqueous solution (12:88).The detection wavelengths for ehlorogenie acid and paeoniflorin were 327 and 230 nm,respectively.The flow rate was 1.0 mL ·min^-1,and the column temperature was 35 22.Results:Chlorogenie acid and paeoniflorin could be separated efficiently under the above ehromatographie condition.The linear ranges of chlorogenic acid and paeoniflorin fell into 0.213 9 -1.283 4 and 0.192 9 -1.157 4 μg,respectively.The regression equations of chlorogenic acid and paeoniflorin were Y =2 733.7X + 154.34 (r =0.999 2)and Y =1 306.8X +23.223 (r =0.999 6),respectively.The average recoveries of ehlorogenie acid and paeoniflorin were 99.51%and 99.62%with RSD values 0.8%and 0.72%.The average contents of chlorogenic acid and paeoniflorin in 6 batches of samples were 0.94 mg·g^-1 and 0.92 mg·g^-1,respectively. Conclusion:This method was simple,accurate,sensitive and repeatable.It can be used for the simultaneous determination of chlorogenic acid and paeoniflorin in Penyanjing capsules.That can provide reference for the quality control of Penyanjing capsule.