小刺猴头菌发酵浸膏多糖体外抗氧化活性分析

In vitro antioxidant activity of polysaccharides extracted from Hericium caput-medusae(Bull.:Fr.)Pers

为研究小刺猴头菌[Hericium caput-medusae(Bull.:Fr.)Pers]发酵浸膏多糖的抗氧化活性。以工厂化小刺猴头菌发酵浸膏为原料,采用透析法分级,DEAE sepharose fast flow柱层析纯化,分别获得粗多糖组分HFCP1和中性多糖HFCP1-1;通过PMP柱前衍生-高效液相色谱法和红外光谱法对单糖组成和结构进行分析,单糖组成均以葡萄糖和半乳糖为主。化学法检测多糖抗氧化活性,结果显示:HFCP1和HFCP1-1清除羟基自由基、超氧阴离子自由基、2,2′-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(2,2′-azinobis-3-ethylbenzthiazoline-6-sulphonate,ABTS+)和1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)的IC50值分别为7.66和6.77mg/mL、6.16和5.30mg/mL、0.63和0.85mg/mL、0.39和2.62mg/mL。以RAW264.7细胞建立H2O2诱导损伤模型,细胞抗氧化结果表明:HFCP1和HFCP1-1处理组与模型组相比均显著增强细胞活力(P<0.05),浓度为100和200mg/L时MDA含量与模型组相比均显著下降(P<0.05);HFCP1浓度为100和200mg/L时与模型组相比显著增加SOD和GSH-Px的活性(P<0.05);HFCP1-1浓度为200mg/L时与模型组相比显著增加SOD活性(P<0.05);也能增加GSH-Px的活性,但未达到显著水平(P>0.05)。

To analyze the antioxidant activity of Hericium caput-medusae(Bull.:Fr.)Pers(HFC),an industrial extraction of H.erinaceus polysaccharide fermentation extract was taken as study object.Using dialysis method and DEAE sepharose fast flow column purification crude polysaccharide components HFCP1 and neutral polysaccharides HFCP1-1 were obtained.The composition and structure of monosaccharides were preliminarily analyzed by PMP precolumn derivatization-high performance liquid chromatography and infrared spectroscopy.The monosaccharides were mainly composed of glucose and galactose.The results showed that the IC50 values of HFCP1 and HFCP1-1 to scavenge hydroxyl radical scavenging,superoxide anion scavenging,ABTS+and DPPH were 7.66 and 6.77,6.16 and 5.30,0.63 and 0.85,0.39 and 2.62 mg/mL,respectively.A H2O2-induced injury model was established in RAW264.7 cells,the results of anti-oxidation of cells showed that the cell viability of HFCP1 and HFCP1-1 treatment group were significantly increased compared with those the model group(P<0.05).The content of MDA was significantly decreased compared with the model group when it concentration was 100 and 200 mg/L(P<0.05).The activities of SOD and GSH-Px were significantly increased compared with those of the model group when HFCP1 concentrations was100 and 200 mg/L(P<0.05).In conclusion,compared with model group,200 mg/L HFCP1-1 significantly increased SOD activity(P<0.05),but also increased GSH-Px activity,but did not reach significant level(P>0.05).