用于微流控芯片的全波长实时荧光检测系统研制

Development of Full-wavelength Real-time Fluorescence Detection System for Microfluidic Chips

为了连续检测出微流控芯片中不同荧光物质的发射光强度,设计了一套基于LabWindows编程的实时的、发射光波长在340-1200 nm的荧光检测系统。系统将控制光谱仪、采集荧光强度、数据降噪及存储、结果分析及显示等功能结合在一起,实现微流控芯片内荧光强度的实时动态检测及分析。系统采用卤钨灯和光谱仪相结合的方式,通过合理设计激发和探测光纤的位置,结合软件算法控制,来消除激发光和背景荧光的影响;系统无需针对不同的荧光物质选用特定的激发光和滤波片,增加系统的集成度、提高检测的多样性。基于本系统对微流控芯片中两种溶液的荧光强度进行检测,来验证系统的测试性能;同时对微流控芯片中不同浓度的荧光物质进行检测,来验证系统的准确性能。实验结果表明所设计的系统满足微流控芯片内不同荧光物质的荧光强度实时检测的要求,为后续基于微流控芯片的生化免疫分析、药物分析和多细胞生命体等研究提供研究基础和分析手段。

In order to continuously detect the emission intensity of different fluorescent substances in the microfluidic chip, a real-time fluorescence detection system with a wavelength of 340-1200 nm was designed based on LabWindows programming. The system combines the functions of the control spectrometer, fluorescence intensity acquisition, data noise reduction and storage, results analysis and display to realize real-time dynamic detection and analysis of fluorescence intensity in the microfluidic chip. The system uses a combination of tungsten halogen lamp and spectrometer to eliminate the influence of excitation light and background fluorescence by rationally designing excitation and detection of the position of the optical fiber and combining software algorithm control;the system does not need to select specific excitation light for different fluorescent substances and Filters increase system integration and increase detection diversity. Based on this system, the fluorescence intensities of the two solutions in the microfluidic chip were tested to verify the test performance of the system. At the same time, the different concentrations of fluorescent substances in the microfluidic chip were tested to verify the accurate performance of the system. The experimental results show that the designed system satisfies the real-time detection requirements of the fluorescence intensity of different fluorescent substances in the microfluidic chip, and provides the basis and analysis for subsequent biochemical immunoassay, drug analysis and multicellular living body research based on the microfluidic chip. means.