摘要
抽穗期是水稻最重要的农艺性状之一,定位和克隆水稻抽穗期QTL具有重要意义。本研究利用早抽品种V20B和晚抽品种CPSLO17作为亲本,构建了150个重组自交系家系(RILs),结合SLAF_seq技术构建的水稻高密度遗传图谱,对水稻抽穗期进行QTL检测。利用MapQTL5进行区间作图,阈值设定为2.5,共检测到2个抽穗期QTL,分别命名为qHD1.1和qHD4.1,这2个QTL的LOD值分别为4.82和3.23,对表型变异的解释率分别为12.8%和8.2%,且增效位点均来自于V20B。本研究为克隆水稻抽穗期QTLs和阐明其分子调控机制提供了依据。
Heading date is one of the most important agronomic traits of rice, and mapping and clone the QTLs controlling the heading date of rice have vital significance. In this study, 150 recombinant inbred lines(RILs) derived from a cross between an earlier heading variety V20 B and a later heading variety CPSLO17 were used to detect rice heading date QTLs, combined with a rice high-density genetic linkage map constructed by SLAF_seq technology. MapQTL5 was used for interval mapping and the threshold was set to 2.5. Two QTLs at heading stage were detected, named qHD1.1 and qHD4.1, respectively. LOD of these two QTLs was 4.84 and 3.23, and the explanatory rates for phenotypic variation were 12.8% and 8.2%, respectively. Moreover, their effect-increasing loci were both originated from V20 B. This study could provide reference for the cloning of QTLs in heading date of rice and the clarification of their molecular regulation mechanism.
作者
姜雪
龙武华
彭强
张习春
刘雪薇
张大双
宫彦龙
张玉珊
朱速松
Jiang Xue;Long Wuhua;Peng Qiang;Zhang Xichun;Liu Xuewei;Zhang Dashuang;Gong Yanlong;Zhang Yushan;Zhu Susong(Guizhou Rice Research Institute,Guizhou Academy of Agricultural Sciences,Guiyang,550006;Guizhou Institute of Bio-technology,Guizhou A- cademy of Agricultural Sciences,Guiyang,550006)
出处
《分子植物育种》
CAS
CSCD
北大核心
2018年第24期8068-8072,共5页
Molecular Plant Breeding
基金
国家重点研发计划项目重点专项(2017YFD0100204)
贵州省科技重大专项(黔科合重大专项字[2013]6023号)
贵州省科研机构服务企业行动计划项目(黔科合服企[2014]4005号)
贵州省科学技术基金项目(黔科合J字[2015]2098号)共同资助
关键词
水稻重组自交系
抽穗期
QTL
Rice recombinant inbred line
Heading date
QTL