紫檀茋影响脂多糖诱导巨噬细胞炎症因子的变化

Effects of pterostilbene on lipopolysaccharide-induced inflammatory cytokine expression in macrophages

采用脂多糖(LPS)诱导小鼠巨噬细胞RAW264. 7建立炎症模型,评价紫檀茋的抗炎作用。在LPS刺激的RAW264. 7细胞中,添加紫檀茋进行干预,采用实时荧光定量聚合酶链式反应(q PCR)方法检测细胞中炎症因子单核细胞趋化蛋白1 (MCP-1),白细胞介素6 (IL-6),白细胞介素1β(IL-1β),肿瘤坏死因子α(TNF-α)和诱导型一氧化氮合酶(iNOS)的mRNA表达量,Griess法检测培养基中一氧化氮(NO)的释放量,采用Western blotting方法进一步检测细胞中细胞外调节蛋白激酶(ERK),c-Jun氨基末端激酶(JNK),p38丝裂原活化蛋白激酶(p38 MAPK)和核转录因子κB-p65 (NF-κB p65)的蛋白磷酸化水平。结果发现:紫檀茋能够显著抑制炎症因子基因的表达和炎症介质NO的释放;紫檀茋+LPS组中ERK,p38和p65的蛋白磷酸化水平显著下降。紫檀茋能显著抑制炎症因子MCP-1,IL-6,IL-1β,TNF-α和iNOS的mRNA表达和NO的释放,其机制可能与阻断丝裂原活化蛋白激酶(MAPK)和NF-κB信号通路相关。

To evaluate the anti-inflammation effect of pterostilbene on RAW264. 7 macrophages, an inflammation model in vitro was established by lipopolysaccharide( LPS) stimulation. After exposing RAW264. 7 cells to pterostilbene and LPS,the mRNA expression of inflammatory cytokines including monocyte chemoattractant protein( MCP)-1,interleukin( IL)-6 and IL-1β,tumor necrosis factor( TNF)-α,and inducible nitric oxide synthase( i NOS) were detected by real-time quantitative polymerase chain reaction( q PCR). Nitric oxide( NO) production in supernatant was analyzed using Griess method. The protein expression of extracellular regulated protein kinases( ERK),c-Jun N-terminal kinase( JNK),p38 mitogenactivated protein kinase( p38 MAPK) and Nuclear factor-κB p65( NF-κB p65) were detected by Western blot analysis. The results showed that pterostilbene significantly inhibited the gene expression of inflammatory cytokines and the production of NO. Pterostilbene significantly suppressed LPS-induced ERK,p38 and p65 phosphorylation. Data showed that pterostilbene could inhibit the mRNA expression of LPS-induced inflammatory cytokines including MCP-1,IL-6,IL-1β,TNF-α and i NOS,and the release of NO,and its mechanism may be related to blocking the mitogen-activated protein kinase( MAPK) and NF-κB pathway.