饲料中添加鼠尾藻粉对大菱鲆幼鱼生长、体成分、抗氧化和非特异免疫参数的影响

Effects of dietary Sargassum thunbergii powder on growth performance,body composition, antioxidation and non-specific immune parameters of juvenile turbot

研究饲料中添加鼠尾藻(Sargassum thunbergii)粉对大菱鲆(Scophthalmus maximus)的影响。在基础饲料中添加0、2. 5%、5%、7. 5%和10%的鼠尾藻粉,饲喂初始体质量(66. 45±0. 52) g的大菱鲆幼鱼8周。结果表明:随着鼠尾藻粉添加量的升高,增重率(WG)、特定生长率(SGR)和日摄食率(DFI)呈现逐渐下降的趋势,2. 5%组与空白组相比无显著差异(P> 0. 05);全鱼粗灰分含量有逐渐升高的趋势,藻粉组均显著高于对照组(P <0. 05);血清低密度脂蛋白胆固醇(LDL-C)、酸性磷酸酶(ACP)和过氧化氢酶(CAT)活性表现出先升高后下降的趋势,在5%组达到最高。本实验条件下,添加2. 5%的鼠尾藻粉对大菱鲆幼鱼的生长无显著影响,添加5%的鼠尾藻粉显著提高了大菱鲆幼鱼的非特异性免疫能力。

This work aimed to appraise the role of dietary seaweed Sargassum thunbergii supplementation in the growth performance,body composition,and immune responsiveness of turbot( Scophthalmus maximus).A basal diet( without supplementation) was supplemented with increasing seaweed powder content to formulate five groups of experimental diets containing 0,2. 5%,5%,7. 5% and 10% of seaweed powder( denoted as ST0,ST2. 5,ST5,ST7. 5,ST10 groups). Turbot juveniles( initial weight 66. 45 ± 0. 52 g)were fed experimental diets for 8 weeks. Results showed that weight gain rate( WGR) and specific growth rate( SGR) tended to decrease with the increasing dietary content of seaweed powder,however,there was no significant differences between ST2. 5 and control group( P > 0. 05),and the other groups were significantly lower than the control group( P < 0. 05). The daily feed intake( DFI) showed a gradually decreasing trend,among which ST10 group was significantly lower than control group( P < 0. 05). Dietary seaweed supplementation had no effect on feed conversion ratio( FCR),survival ratio( SR),viscerosomatic index( VSI) and hepatosomatic index( HSI). Crude protein content of whole fish tended to increase gradually,and crude fat content increased firstly and then decreased,however,both had no significant differences among groups( P > 0. 05). Crude ash content increased gradually( P < 0. 05),which was consistent with the trend of crude ash content of feedstuff. The concentration of serum low density lipoprotein cholesterol( LDL-C)showed a trend of first increase and then decrease( P < 0. 05),and reached the highest value at ST5 group.Serum acid phosphatase( ACP) and catalase( CAT) activity increased first and then decreased,and also reached the highest value at ST5 group. Under the present experimental conditions,the addition of 2. 5% S.thunbergii powder had no significant effect on juvenile turbot growth. Adding 5% S. thunbergii powder significantly increased the non-specific immune capacity of juvenile turbot.