植入式遥测技术在抑郁后大鼠室性心律失常中的应用

Application of implantable telemetry in ventricular arrhythmia in rats after depression

目的用植入式遥测技术(DSI)研究抑郁后大鼠室性心律失常的发生机制。方法将20只SD大鼠随机数字表法分成正常组(n=10)和抑郁组(n=10)。抑郁组大鼠经过28d慢性不可预见温和应激(CUMS)建立抑郁模型,正常组不给任何刺激正常饲养28d。糖水消耗实验、强迫游泳实验评价抑郁模型制作。应用植入式遥测技术研究抑郁与室性心律失常的关系。结果与正常组相比,抑郁组糖水消耗量明显减少[(38.3±14.8)%对(64.4±12.7)%,P<0.05],强迫游泳不动时间明显增加[(18.6±14.3)s对(115.8±24.4)s,P<0.05]。与正常组相比,抑郁后室性心律失常评分明显增加[(46.20±10.89)对(6.80±1.72),P<0.01]抑制交感神经后,抑郁组心率的波动幅度更大[(-83.6±13.7)次/min对(-45.9±6.3)次/min,P<0.05]。心率变异性(HRV)分析表明抑郁组低频(LF)、LF/高频(HF)较正常组显著升高(P<0.05)。线性回归发现LF(r2=0.687,P<0.001)、LF/HF(r2=0.756,P<0.001)与室性心律失常的发生呈正相关。同时,抑郁组血浆中肾上腺素[(5029±1476)pg/ml对(1032±389)pg/ml,P<0.01]以及去甲肾上腺素[(40.2±3.5)ng/ml对(13.5±3.8)ng/ml,P<0.01]水平均明显高于正常组。结论交感神经过度激活增加抑郁后室性心律失常发生的风险。

Objective To investigate the mechanism of ventricular arrhythmias in rat model of depression by using the implanted telemetry electrocardiogram methods. Methods Twenty SD rats were randomly divided into normal group (n=10) and depression group (n=10). The depression group was established by 28 days of chronic unpredictable mild stress (CUMS). The normal group was given no stimulation for normal feeding for 28 days.Glucose consumption experiment and forced swimming experiment were used to evaluated depression model procedure.Implanted telemetry electrocardiogram was used to evaluated the sympathetic nerve activity. Results Compared with the normal group, the consumption of glucose in the depression group was significantly decreased[(38.3±14.8)% vs. (64.4±12.7)%, P<0.05], and the stable time of forced swimming was significantly increased[(18.6±14.3)s vs. (115.8±24.4)s), P<0.05]. Ventricular arrhythmia score in the depression group was significantly higher compared with normal group[(46.20±10.89)vs.(6.80±1.72), P<0.01]. After suppression of sympathetic nerve, the fluctuation of heart rate in the depression group was greater [(-83.6±13.7) bpm vs.(-45.9±6.3) bpm, P<0.05]. Heart rate variability(HRV)analysis showed that low frequency(LF) and LF/high frequency(HF) were significantly increased in the depression group (P<0.05). Linear regression found that LF (r2=0.687, P<0.001) and LF/HF (r2=0.756, P<0.001) were positively correlated with the occurrence of ventricular arrhythmia.Furthermore, the levels of epinephrine[(5 029±1 476) pg/ml vs.(1 032±389)pg/ml, P<0.01) and norepinephrine[(40.2±3.5) ng/ml vs.(13.5±3.8) ng/ml, P<0.01]in depression group were significantly higher than normal group. Conclusion Sympathetic hyperactivity increases the risk of ventricular arrhythmias in depressive model rats.