摘要
该文旨在研究光强对垂盆草生长发育、活性成分积累及体外抗氧化活性的影响,探索高产优质垂盆草的光强条件。设置5个光强处理,分别为全光照的100%(G1),77%(G2),60%(G3),38%(G4)和16%(G5),收获时测定生长指标、产量、多种活性成分含量及体外抗氧化活性。结果表明,随着光照强度降低,垂盆草最大分枝长与平均节间距增大。G2处理显著提高垂盆草叶片层数、分枝数,G3处理显著提高垂盆草生物量。总黄酮和总酚含量以G3处理最高。各处理槲皮素和异鼠李素含量差异达到显著水平,均以G1处理最高,G2,G3处理次之。各处理山柰素含量无显著差异。垂盆草水提物清除DPPH自由基、抑制脂质过氧化及还原能力均以G1处理较强。综合比较垂盆草药材产量与活性成分含量和抗氧化活性,光照强度不低于60%利于垂盆草高产优质兼得。
The present study was conducted to explore the effect of light intensity on growth,bioactivity compounds accumulation and anti-oxidative activity of Sedum sarmentosum. The growth,yield,contents of total flavonoids,total phenolic,quercetin,kaempferol and isorhamnetin,and antioxidant activities were assessed in S. sarmentosum under five light intensities,namely 100% full sunlight(G1),77% full sunlight(G2),60% full sunlight(G3),38% full sunlight(G4),and 16% full sunlight(G5). The results showed that light intensity significantly affected the growth and the chemical compounds accumulation. With the decrease of light intensity,the maximum branch length and the average internode distance increased. G2 treatment greatly promoted the numbers of leaf layers and branches,and G3 treatment remarkably improved the yield. The highest total flavonoids and phenolic contents were obtained in G3 treatment. Meanwhile,the highest quercetin and isorhamnetin contents were obtained in G1 treatment. The difference of kaempferol content was not significant. In addition,based on DPPH,FTC and FRAP methods,the antioxidant activities of the aqueous extracts under G1 treatment were superior to the others. The results indicated that more than 60% full sunlight was the optimum light intensity condition to achieve high yield and quality of S. sarmentosum.
作者
朱再标
杨金凤
郭巧生
刘帆
王蓉
张文霞
ZHU Zai-biao;YANG Jin-feng;GUO Qiao-sheng;LIU Fan;WANG Rong;ZHANG Wen-xia(Institute of Chinese Medicinal Materials,Nanjing Agricultural University,Nanfing 210095,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2018年第22期4404-4409,共6页
China Journal of Chinese Materia Medica
基金
中央高校基本科研业务费专项资金项目(KYZ201734)