辣木叶的UPLC特征指纹图谱研究

UPLC characteristic fingerprint of leaves of Moringa oleifera

建立辣木叶的超高效液相(UPLC)特征指纹图谱分析方法,为辣木叶质量控制提供科学依据。采用UPLC建立辣木叶的特征指纹图谱,色谱柱为Agilent Eclipse XDB-C18(3. 0 mm×100 mm,1. 8μm),流动相为乙腈-0. 01%三氟乙酸水溶液,梯度洗脱,流速为0. 5 m L·min^-1,检测波长为210 nm,柱温为35℃。采用"中药色谱指纹图谱相似度评价系统2004A版"对14批辣木叶样品进行相似度分析以比较各个批次样品间的一致性。建立了辣木叶的UPLC特征指纹图谱,确定了12个共有峰,并根据对照品和UPLC-MS对这12个色谱峰进行结构确认,其相对保留时间为0. 08 (峰1,腺苷),0. 14 (峰2,L-苯丙氨酸),0. 22 (峰3,5-咖啡酰奎宁酸),0. 28 (峰4,L-色氨酸),0. 42 (峰5,4-咖啡酰奎宁酸),0. 65 (峰6,维采宁-2),0. 94 (峰7,牡荆素),0. 96 (峰8,异牡荆素),1. 00 (峰9,异槲皮素),1. 11 [峰10,槲皮素3-O-(6″-丙二酰基)-β-D-葡萄糖苷],1. 21(峰11,紫云英苷),1. 37 [峰12,山柰酚3-O-(6″-丙二酰基)-β-D-葡萄糖苷]。该方法首次建立了辣木叶的UPLC特征指纹图谱,方法简便、快速、准确性高、重复性好,可为辣木叶的质量评价和控制提供科学依据。

This study aims to establish the characteristic fingerprint of the leaves of Moringa oleifera by Ultra High Performance Liquid Chromatography(UPLC) for its quality control. The method was developed on a column of Agilent Eclipse XDB-C18 with acetonitrile-0. 01% TFA solution as the mobile phase by gradient elution at a flow rate of 0. 5 m L·min^-1. The detective wavelength was 210 nm,and the column temperature was 35 ℃. The 14 batches of the leaves of M. oleifera were compared for the similarity by using Traditional Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System(2004 A). The UPLC characteristic fingerprint was established,and twelve common peaks were identified by comparison with the references and UPLC-MS. The relative retention times were 0. 08(No. 1,adenosine),0. 14(No. 2,L-phenylalanine),0. 22(No. 3,5-caffeoylquinic acid),0. 28(No. 4,L-tryptophane),0. 42(No. 5,4-caffeoylquinic acid),0. 65(No. 6,vicenin-2),0. 94(No. 7,vitexin),0. 96(No. 8,isovitexin),1. 00(No. 9,isoquercitrin),1. 11 [No. 10,quercetin 3-O-β-D-(6″-malonyl)-glucopyranoside],1. 21(No. 11,astragalin) and 1. 37[No. 12,kaempferol 3-O-β-D-(6″-malonyl)-glucopyranoside]. It is the first time to establish the UPLC characteristic fingerprint of the leaves of M. oleifera. The method is simple,quick and reproducible with high precision,which can provide a scientific basis for the quality control of the leaves of M. oleifera.