孔圣枕中丹对2型糖尿病大鼠认知功能的影响

Effects of Sagacious Confucius’ Pillow Elixir on cognitive function in type 2 diabetic rats

2型糖尿病(type 2 diabetes mellitus,T2DM)是目前威胁人类健康的主要因素,且患者常伴随严重的认知功能障碍。孔圣枕中丹为中医治疗健忘的经典方剂,该研究通过STZ诱导SD大鼠建立T2DM模型,通过比较学习记忆能力、血糖水平、血清胰岛素含量、海马神经元病理改变、β-淀粉样蛋白(Aβ)含量及Tau蛋白的磷酸化程度等,探讨孔圣枕中丹改善T2DM大鼠认知障碍的作用机制。结果表明,孔圣枕中丹能显著改善大鼠的学习记忆功能,降低Aβ蛋白在海马及血清中的含量以及血清胰岛素含量,改善海马神经元的受损情况,有效降低Tau蛋白的磷酸化程度。对血糖、胰岛素等基础指标也有一定的改善作用。结果表明,孔圣枕中丹对T2DM大鼠海马病理改变和神经损伤具有改善作用,有望为人类T2DM所致认知功能障碍的临床辅助治疗提供实验依据。

Type 2 diabetes mellitus(T2 DM) is the leading threat to human health in China,and severe cognitive impairment often occurs in most T2 DM patients. Although Sagacious Confucius’ Pillow Elixir is a type of classical traditional Chinese medicine for cognitive impairment in clinic,the mechanism has not yet been completely defined. In this study,an experimental model of type 2 diabetes mellitus(T2 DM) was established by injecting Sprague Dawley(SD) rats with streptozocin(STZ),so as to compare the learning and memory ability,hippocampal neurons pathological changes,beta amyloid protein(A beta) content,degree of Tau protein phosphorylation,blood glucose and insulin level. The results showed that the Sagacious Confucius’ Pillow Elixir could improve the learning and memory ability of STZ injected rats,reduce the level of A beta content both in hippocampus and serum,effectively reduce Tau protein phosphorylation degree,and also significantly alleviate hippocampal pathological injury,blood glucose,insulin and other basic indicators. The results showed that Sagacious Confucius’ Pillow Elixir can alleviate the hippocampal pathological damage caused by STZ,and is expected to provide a theoretical basis for human T2 DM patients in clinical adjuvant therapy.