高温环境下蚂蟥病害病原初探

Preliminary study on pathogen of Whitmania pigra disease in high temperature environment

该文以健康蚂蟥为对照利用高通量测序对高温环境下患病蚂蟥病变肌肉、肠道、发病蚂蟥养殖水体环境与沉积物的细菌进行16S rDNA序列测定,分析细菌多样性,分析高温环境下患病蚂蟥细菌性疾病的致病菌。结果表明,各处理原始序列达83 000以上,并且有效序列占比在87%以上,GC量在52%~54%,细菌多样性丰富。细菌相对丰度分析表明各处理中均以变形菌门、拟杆菌门、厚壁菌门细菌含量最为丰富。与健康蚂蟥肌肉、肠道相比,发病蚂蟥肌肉、肠道中拟杆菌属、假单胞菌属、脱硫弧菌属细菌相对丰度显著升高,且在发病蚂蟥养殖水体和沉积物中具有丰富的含量,推测诱发蚂蟥夏季高温细菌性疾病的致病菌为拟杆菌属、假单胞菌属、脱硫弧菌属细菌。

In this paper,on the contrast of healthy leech,the bacterial diversities were analyzed by 16S rDNA sequence analysis of the bacteria of muscle and intestinal tract of Whitmania pigra,the environment water and sediment of cultivating the diseased Wh.pigra in high temperature by high-throughput sequencing to determine the possible pathogenic bacteria of bacterial diseases of Leech in high temperature. The results showed that the original sequence reached over 83 000,and the effective sequences accounted for more than 87%. The GC contents ranged from 52% to 54% and the bacterial diversities were abundant. Bacterial relative abundance analysis showed that the bacterial content of Proteobacteria,Bacteroidetes,and Firmicutes was the most abundant in all treatments. Compared with healthy leech muscles and intestines,the muscle and intestinal tract of pathogenic leech relative abundance of Bacteroides,Pseudomonas,and Desulfovibrio was significantly increased,and it was abundant in water and sediment of diseased leeches,Lead to the possibility that the pathogenic bacteria of this bacterial disease may be Bacteroides,Pseudomonas,Desulfovibrio.