蚂蟥性腺发育周期内性类固醇激素变化初步研究

Preliminary study on changes of sex steroid hormones in periodic development of Whitmania pigra gonad

为了解蚂蟥性腺发育周期内性类固醇激素变化规律,采用酶解、萃取以及UPLC-MS/MS技术测不同月龄蚂蟥体内性类固醇激素含量变化趋势。结果表明:蚂蟥体内雌酮、雌三醇、睾酮和孕酮含量均呈M型变化趋势,均在6月龄达最大值,9月龄最低,10~11月龄开始轻微上升,其中睾酮和孕酮另一个小峰值均出现在2月龄,而雌酮和雌三醇均出现在8月龄。1~4月龄睾酮和孕酮含量稍高于雌三醇,5月龄开始雌酮和雌三醇激素含量稍高于睾酮和孕酮。综上,蚂蟥体内性类固醇激素含量随性腺成熟呈逐渐上升、排放期后迅速下降趋势,提示蚂蟥雌激素、雄激素和孕激素之间的比例可能与其性腺发育的特殊性存在极大相关性。

The present study was conducted to investigate the rule of sex steroid hormones dynamic in the periodic development of Whitmania pigra gonad. The dynamic of sex steroid hormones in different age of Wh. pigra were detected by enzymolysis,extraction and UPLC-MS/MS. The results showed that the concentrations of estrone,estriol,testosterone and progesterone in the Wh. pigra showed M-type curve and peaked in 6-month-old Wh. pigra. The concentrations of above-mentioned four steroid hormones were the lowest in 9-month-old and then increased slightly. The another peaks of testosterone and progesterone were found in 2-month-old and the another peaks of estrone and estriol were obtained in 8-month-old. The concentrations of testosterone and progesterone are slightly higher than estriol during 1-month-old to 4-month-old,and thereafter strone and estriol showed higher concentration than testosterone and progesterone. In summary,the concentrations of four sex steroid hormones in Wh. pigra increased gradually with the maturation of gonads and decreased rapidly after the discharge period,which indicated that the ratio between estrogen,androgen and progesterone may be greatly related to the specificity of gonad development.