维吾尔族药大戟脂中三萜类化学成分研究

Triterpenoids from Uygur medicine latex of Euphorbia resinifera

该研究通过反复硅胶柱色谱、ODS柱色谱和半制备型HPLC等方法,从维吾尔族药大戟脂的甲醇超声提取物中共分离得到10个三萜类化合物。利用各种波谱学手段及理化性质确定了这些化合物的结构,分别鉴定为3β-hydroxy-25,26,27-trinoreupha-8-ene-24-oate(1)、iso-maticadienediol(2)、25,26,27-trinortirucall-8-ene-3β-ol-24-acid(3)、dammarendiolⅡ(4)、eupha-8,24-diene-3-ol-26-al(5)、lnonotusane C(6)、eupha-8,24-diene-3β-ol-7,11-dione(7)、inoterpene A(8)、inoterpene B(9)和eupha-24-methylene-8-ene-3β-ol-7,11-dione(10),其中化合物1为新天然产物,化合物2~4首次从该科植物中分离得到,化合物5和6首次从该属植物中分离得到。细胞毒活性测试结果显示化合物1~10对MCF-7,U937以及C6细胞株均未表现出明显的细胞毒活性。

Ten triterpenes compounds were isolated from the methanol extraction of the latex of Euphorbia resinifera by means of various chromatographic methods such as silica gel,ODS and semi-preparative HPLC,Their structures were identified by spectroscopic methods and physicochemical properties. These isolated compounds were identified as 3β-hydroxy-25,26,27-trinor eupha-8-ene-24-oate( 1),iso-maticadienediol( 2),25,26,27-trinor Tirucall-8-ene-3β-ol-4-acid( 3),dammarendiol Ⅱ( 4),eupha-8,24-diene-3-ol-26-al( 5),lnonotusane C( 6),eupha-8,24-diene-3β-ol-7,11-dione( 7),inoterpene A( 8),inoterpene B( 9),and eupha-24-methylene-8-ene-3β-ol-7,11-dione( 10). Among them,compound 1 was a new natural product,compounds 2-4 were firstly isolated from the Euphorbiaceae and compounds 5 and 6 were isolated from the genus Euphorbia for the first time. The cytotoxicity of the compounds 1-10 against MCF-7,U937 and C6 cancer cell lines was evaluated,but none of the compounds was active.