单叶蔓荆子的化学成分研究

Chemical constituents from fruits of Vitex trifolia var. simplicifolia

对马鞭草科牡荆属植物单叶蔓荆Vitex trifolia var.simplicifolia果实中的化学成分进行研究。通过反复硅胶柱色谱、ODS柱色谱、Sephadex LH-20凝胶柱色谱和制备型高效液相等方法对其提取物进行分离和纯化,并利用核磁共振(NMR)以及质谱等现代波谱学技术对分离得到的化合物的进行结构鉴定。从单叶蔓荆的干燥成熟果实的乙醇提取物的氯仿萃取部位和乙酸乙酯萃取部位中分离得到了18个化合物,分别鉴定为ent-2-oxo-15,16,19-trihydroxy-pimar-8(14)-ene(1)、猫眼草酚D(2)、紫花牡荆素(3)、木犀草素(4)、泽兰黄素(5)、芹素菜(6)、5,4’-dihydroxy-3,6,7-trimethoxyflavone(7)、luteolin-4’-O-glucoside(8)、hypolaetin-7-O-β-D-glucopyranoside(9)、当药黄素(10)、agestricin D(11)、5,3’-dihydroxy-6,7,4’-trimethoxy-flavanone(12)、委陵菜酸(13)、2α,3β,23-trihydroxyolean-12-en-28-oic acid(14)、3’-acetoxy-4’-angeloyloxy-3’,4’-dihydroseselin(15)、dihydrodehydrodiconiferyl alcohol(16)、3,5’-dimethoxy-4’,epoxy-8,3’-neolignane-5,9,9’-triol(17)和salicifoliol(18)。化合物1,2,5~15,17,18为首次从该植物中分离得到,其中化合物1,5,7~11,15,17,18为首次从该属植物中分离得到。

The present study is to investigate the chemical constituents from the dried ripe fruits of Vitex trifolia var. simplicifolia The compounds were isolated by using a variety of chromatographic methods including silicagel,ODS,Sephadex LH-20,reversedphase HPLC,and other methods. Their structures were identified by NMR,and MS date. As a result,18 compounds were isolated and identified as ent-2-oxo15,16,19-trihydroxypimar-8( 14)-ene( 1),chrysosplenol D( 2),casticin( 3),luteolin( 4),eupatrin( 5),apigenin( 6),5,4’-dihydroxy-3,6,7-trimethoxyflavone( 7),luteolin-4’-O-glucoside( 8),hypolaetin-7-O-β-D-glucopyranoside( 9),swertisin( 10),agestricin D( 11),5,3’-dihydroxy-6,7,4’-trimethoxyflavanone( 12),tomentic acid( 13),2α,3β,23-trihydroxyolean-12-en-28-oic acid( 14),3’-acetoxy-4’-angeloyloxy-3’,4’-dihydroseselin( 15),dihydrodehydrodiconiferyl alcohol( 16),3,5’-dimethoxy-4’,7-epoxy-8,3’-neolignane-5,9,9’-triol( 17) and salicifoliol( 18). Among them,compounds 1,2,5-15,17 and 18 were obtained from V. trifolia var. simplicifolia Cham for the first time and compounds 1,5,7-11,15,17 and 18 were isolated from thegenus Vitex for the first time.