纤维蛋白原的^(131)I标记

^(131)I Labeling of Ribrinogen

目的通过研究131I-纤维蛋白原在体内的留滞时间,提出一种简单高效的蛋白质放射性碘标记的方法。方法利用Iodogen(四氯二苯基甘尿)为氧化剂对纤维蛋白原进行的131I标记,取样并测定标记率及生物活性。结果分别使用5、10、20μg lodogen氧化剂反应20 min后测定的标记率为89. 2%、88. 4%、88. 7%。使用10μg的lodogen氧化剂进行氧化标记测得的5、15、30 min的标记率为64. 3%、89. 6%和88. 2%,生物活性率为86. 1%、84. 7%和85. 5%。结论以Iodogen为氧化剂,一步反应,即可获得纤维蛋白原碘标记物。标记率及生物活性率都接近90%。

Objective A simple and efficient method for protein radioactive iodine labeling was proposed by studying the retention time of 131 I-fibrinogen in vivo. Methods Iodogen was used to mark the 131 I for fibrinogen,sampling and determining the marking rate and biological activity. Results After 20 minutes of reaction,the marker rates were 89. 2%,88. 4%and 88. 7% for 5 μg,10 ug and 20 μg of Iodogen oxidants,respectively. using,. By using 10 μg of Iodogen oxidants,the mark rates were 64. 3 %,89. 6% and 88. 2%,and the biologically active rates were 86. 1%,84. 7% and 85. 5% after 5 minutes,15 minutes and 30 minutes of reactions,respectively. Conclusion By using Iodogen as an oxidant,one step reaction can obtain the fibrinogen iodide marker. Both the marking rate and the biologically active rate are close to 90%.