白鲜根皮的化学成分及其细胞毒活性研究

Chemical constituents from root barks of Dictamnus dasycarpus and their cytotoxic activities

综合运用多种现代色谱分离方法对白鲜根皮中的化学成分进行研究,从白鲜根皮的甲醇提取物中分离得到19个化合物。采用UV,IR,NMR,MS和CD等波谱方法鉴定了这些化合物的化学结构,分别为kihadanin D(1)、黄柏酮(2)、kihadanin A(3)、kihadanin B(4)、kihadanin C(5)、柠檬苦素(6)、吴茱萸内酯醇(7)、梣酮(8)、furo[2,3-b]quinolin-4-ol (9)、前茵芋碱(10)、ifflaiamine(11)、白鲜醇(12)、柚皮素(13)、香叶木素(14)、汉黄芩素(15)、东莨菪素(16)、黄花菜木脂素A(17)、香草酮(18)和焦谷氨酸甲酯(19),其中化合物1为新的柠檬苦素类化合物,化合物9为首次从植物界分离得到,化合物11,13~14,17~19为首次从该属植物中分离得到。对化合物1~18的细胞毒活性进行了筛选,发现1对人乳腺癌细胞(MDA-MB-231),人非小细胞肺癌细胞(A549)和人结肠癌细胞(HT29)具有一定生长抑制作用,IC50分别为16. 22,21. 72,31. 06μmol·L-1。

Nineteen compounds,including kihadanin D( 1),obacunone( 2),kihadanin A( 3),kihadanin B( 4),kihadanin C( 5),limonin( 6),evodol( 7),fraxinellone( 8),furo[2,3-b]quinolin-4-ol( 9),preskimmianine( 10),ifflaiamine( 11),dictamnol( 12),naringenin( 13),diosmetin( 14),wogonin( 15),scopoletin( 16),cleomiscosin A( 17),apocynin( 18),and methyl pyroglutamate( 19),were isolated from the methanol extract of the root barks of Dictamnus dasycarpus by using various column chromatographies. Their chemical structures were extensively determined on basis of UV,IR,NMR,MS,and CD spectroscopic data analyses.Among them,1 is a new limonoid,9 was isolated from plant kingdom for the first time,11,13-14 and 17-19 were obtained from the genus Dictamnnus for the first time. Cytotoxicities of compounds 1-18 were tested,and the results indicated that 1 exhibited cytotoxicities against three human cancer cell lines MDA-MB-231,A549 and HT29 with IC50 values of 16. 22,21. 72 and 31. 06 μmol·L-1,respectively.