基于网络药理学的淫羊藿防治疾病的优效组分及信号通路研究

Effective components and signaling pathways of Epimedium brevicornum based on network pharmacology

该研究采用网络药理学技术筛选出中药淫羊藿防治疾病的活性组分,并对潜在的靶点与机制进行整合与分析。采用TCMSP数据库筛选活性成分,利用TTD和DrugBank数据库配合文献挖掘潜在靶点进行预测,利用通路注释技术对淫羊藿中活性成分及潜在靶点进行通路的富集与分析。该研究共筛选出淫羊藿中具有潜在靶点的活性成分34个,主要包括淫羊藿苷,朝藿定A,朝藿定B,朝藿定C,淫羊藿A,淫羊藿C在内的21个黄酮类组分;(+)-cycloolivil和olivil在内的木脂素类组分以及谷甾醇、24-epicampesterol和poriferast-5-en-3beta-ol在内的甾醇类组分。另外,筛选出与之相对应的作用靶点183个,主要包含Ptgs2,NCOA6,RANK,OPG,WNT9B,PTH1R,BMPs,SMAD4A等;共涉及信号通路84条,如NF-κB信号通路、T细胞受体信号通路、IL-17信号通路等在内的8条炎症相关通路,乳腺癌、膀胱癌、胰腺癌在内的10条肿瘤疾病类相关通路以及与雌激素信号通路在内的内分泌等相关通路。这为淫羊藿活性组分的发现及作用机制研究奠定了基础。

The aim of this paper was to find out the active components of Epimedium brevicornum using network pharmacology, and find the potential targets and mechanisms. The TCMSP database was used to screen the active ingredients, and TTD and DrugBank databases were used to predict the potential targets with the literature mining. The pathway annotation was used to enrich and analyze the active ingredients and potential targets of E. brevicornum. The results showed that E. brevicornum had34 potential target active ingredients, including 21 flavones components, such as icariin, epimedin A, epimedin B, epimedin C, Yinyanghuo A, Yinyanghuo C and so on, 2 lignans involved in(+)-cycloolivil and olivil, 3 sterols consisting of sitosterol, 24-epicampesterol and poriferast-5-en-3 beta-ol. The main predicted targets included Ptgs2, NCOA6, RANK, OPG, WNT9 B, PTH1 R, BMPs, SMAD4 A and so on. There were 88 signaling pathways involved in 10 signaling pathways which was related to inflammation, such as NF-kappa B signaling pathway, T cell receptor signaling pathway, IL-17 signaling pathway and 10 pathways which was related to cancer included breast cancer, bladder cancer, pancreatic cancer and so on, and estrogen related signaling pathways included estrogen signaling pathway. This laid the foundation for the discovery of the active components of Epimedium and the study on its mechanism of action.