内皮型一氧化氮合成酶T786C、白细胞介素-6C572G基因多态性与动脉瘤性蛛网膜下腔出血的相关性研究

The relationship between polymorphisms of endothelial tyrosineoxide synthase T786C,interleukin-6 C572G and aneurysmal subarachnoid hemorrhage

目的探讨eNOS T786C、IL-6 C572G单核苷酸多态性与动脉瘤性蛛网膜下腔出血的相关性。方法收集2014年1月-2016年2月就诊于内蒙古包头市中心医院的动脉瘤蛛网膜下腔出血患者71例及非蛛网膜下腔出血或健康人群142例基线资料[实验组与对照组1:2,性别相同,年龄35～81岁,平均年龄(55.07±9.98)岁],采集抗凝血标本,应用聚合酶链式反应(PCR)和限制性片段长度多态性(RFLP)分析方法检测实验组及对照组基因型,实验组和对照组之间基因型分布和等位基因频率分布比较采用χ~2检验,明确各基因型与散发颅内破裂动脉瘤蛛网膜下腔出血疾病之间的相关性。结果实验组与对照组eNOS T786C、IL-6 C572G各基因型、等位基因无明显差异(P>0.05);实验组与对照组高血压病、长期吸烟人数有明显差异(P<0.05),其余指标均无明显差异(P>0.05)。结论 eNOS T786C、IL-6 C572G基因多态性与破裂的动脉瘤性蛛网膜下腔出血无关。

Objective To study the relationship between eNOS T786C and IL-6 C572G single nucleotide polymorphisms and the formation of intracranial aneurysms.Methods 71 patients (age≥15 years)with spontaneous SAH were identified in Baotou central hospital from January 2014 to February 2016.For each case,two controls without SAH were matched according to gender,5-year age strata and sector of residence in Baotou.Controls were family members or friends of other patients with non-neurological conditions in Baotou central hospital.Data on medical history and lifestyle factors were collected from cases and controls by interview of the subject or a proxy informant.Anti-acquisition blood samples were extracted whiter blood cells from peripheral blood by lymphocyte separation liquid,extracted venous blood genomic DNA sample was tested by using DNA kit,made the restriction enzyme digestion and sequenced the PCR products.The genotype distribution and allele frequency distribution between the experimental group and the control group were compared by χ~2 test.The relationship between genotypes and subarachnoid hemorrhage of sporadic ruptured intracranial aneurysms was determined.Results eNOS T786C and IL-6 C572G gene polymorphisms had no significant difference between the experimental group and the control group (P>0.05);There was significant difference in the number of hypertension and long-term smoking between the experimental group and the control group (P<0.05),but there was no significant difference in the other parameters between the experimental group and the control group (P>0.05).Conclusion eNOS T786C and IL-6 C572G gene polymorphisms might not be the risk actors for the formation of intracranial aneurysms.