三氧化二砷联合小白菊内酯诱导人骨肉瘤细胞凋亡的实验研究

Experimental study on apoptosis of human osteosarcoma cells induced by arsenic trioxide combined with Parthenolide

目的研究三氧化二砷与小白菊内酯联合应用于人骨肉瘤细胞U2OS,对其增殖产生的抑制作用。方法培养人骨肉瘤细胞株U2OS作为研究对象,选取不同药物浓度的三氧化二砷及小白菊内酯分别以单独和联合应用的形式作用于骨肉瘤细胞后,通过倒置显微镜观察其细胞学形态的变化,应用MTS方法分析药物对肿瘤细胞增殖的抑制作用。结果三氧化二砷和小白菊内酯联合用药对人骨肉瘤细胞U2OS具有生长抑制作用,并优于三氧化二砷或小白菊内酯单独用药,而且小白菊内酯及三氧化二砷对U2OS细胞抑制作用具有时间和浓度依赖性。结论三氧化二砷和小白菊内酯联合用药诱导人骨肉瘤细胞U2OS的凋亡,起到了良好的抗肿瘤作用。

Objective To study the inhibitory effect of arsenic trioxide(ATO)and Parthenolide(PTL)on the proliferation of human osteosarcoma cell U2OS.Methods Arsenic trioxide and parthenolide were used to treat the U2OS osteosarcoma cells individually or jointly by diffrernt drug concentration.The changes of cellular morphology were observed by inverted microscope,analysis of inhibitory effects of drugs on tumor cells by MTS method.Results The combination of arsenic trioxide and parthenolide inhibited the growth of human osteosarcoma cell line U2OS much better than each agent alone.The results showed that parthenolide and arsenic trioxide exhibited concentration-dependent and time-dependent inhibitory effect for U2OS osteosarcoma cells.Conclusion The combination of arsenic trioxide and parthenolide can induce the apoptosis of human osteosarcoma cell line U2OS,and it has a good antitumor effect.