艾灸预处理“天枢”穴对溃疡性结肠炎大鼠肠黏膜屏障保护的机制研究

Protective Mechanism of Preventative Moxibustion at Tianshu Acupoint(ST25) on Intestinal Barrier in UC Rats

目的:观察艾灸预处理"天枢"穴对UC大鼠的抗炎效应,探讨艾灸预处理"天枢"穴对UC大鼠肠黏膜屏障的保护机制。方法:SD雄性大鼠28只,随机分为正常组、模型组、隔药灸预处理组和温和灸预处理组,每组7只。造模前先予隔药灸、温和灸预处理干预7天;预处理后予模型组、隔药灸预处理组、温和灸预处理组4%葡聚糖硫酸钠水溶液连续饮用7天。采用HE染色法观察大鼠结肠组织病理学变化;免疫组化法和western blot法检测大鼠结肠组织分子的蛋白表达。结果:与正常组比较,模型组结肠组织病理学评分显著升高,Occludin、JAM1、MUC2、ZO-1蛋白表达显著降低,NF-κBp65、IL-1β蛋白表达显著升高(P <0.05);与模型组比较,隔药灸预处理组结肠组织病理学评分显著降低,Occludin、MUC2、JAM1、ZO-1蛋白表达显著升高,NF-κBp65、IL-1β蛋白表达显著降低(P <0.05),温和灸预处理组结肠组织病理学评分显著降低,Occludin、MUC2、ZO-1蛋白表达显著升高,IL-1β蛋白表达显著降低(P <0.05)。结论:隔药灸预处理、温和灸预处理"天枢"穴均能升高UC大鼠肠黏膜屏障相关蛋白的表达,可能是艾灸有效预防减轻UC的机制之一。

Objective: To observe the effect of anti-inflammatory by preventative moxibustion at Tianshu Acupoint(ST25)on UC rats, and to investigate the protective mechanism of preventative moxibustion at Tianshu Acupoint(ST25) onintestinal mucosal barrier in UC rats. Methods: A total of 28 male Sprague-Dawley rats were randomly divided into thenormal group(NC), the ulcerative colitis group(UC), the herb-partitioned moxibustion pretreatment group(HM+P), thewarming moxibustion pretreatment group(WM+P), with 7 in each group. The HM+P and the WM+P were treated withherb-partitioned moxibustion and warming moxibustion for 7 days before the model was established. After pretreatment,the UC, the HM+P and the WM+P were given 4% dextran sodium sulfate solution for 7 days. Observe the rat pathologicalchanges of colon tissue by HE staining, detect the protein expression in rat colon tissue by immunohistochemistry andwestern blot. Results: compared with the NC group, the pathological scores were increased, the expression of occludin,JAM1, MUC2, ZO-1 were decreased and the expression of NF-κBp65, IL-1βincreased in UC group(P < 0.05);compared with the UC group, the pathological score decreased, the protein expression of occludin, JAM1, MUC2,ZO-1 increased, the protein expression of NF-κBp65, IL-1βdecreased in HM + P group(P < 0.05), the Pathological scoredecreased, the Protein expression of occludin, MUC2, ZO-1 increased, the Protein expression of IL-1β decreased inWM + P group(P < 0.05). Conclusion: The herb-partitioned moxibustion pretreatment and the warming moxibustionpretreatment could increase the expression of intestinal barrier related proteins in UC rats, it may be one of themechanisms of moxibustion to relieve colonic inflammation of UC.