越鞠丸对Balb/c小鼠和C57BL/6J小鼠抗抑郁作用与前额叶场电位差异性分析

Analysis of Differences of Antidepressant Effect of Yueju Pill between Balb/c Mice and C57BL/6J Mice

目的:比较Balb/c小鼠和C57BL/6J小鼠中越鞠丸抗抑郁作用的差异并分析其对小鼠前额皮层(prefontal cortex,PFC)突触传递的影响。方法:成年健康的雄性Balb/c小鼠和C57BL/6J小鼠随机分为对照组和越鞠丸组,给药30 min后进行强迫游泳测试(forced swimming test,FST),运用电生理实验(electrophysiologicalexperiment)检测小鼠前额皮层的场电位(field excitatory postsynaptic potential,fEPSP)及长时程增强(long termpotentiation,LTP)。结果:强迫游泳测试中,Balb/c小鼠的越鞠丸组不动时间较对照组明显降低(P <0.01),C57BL/6J小鼠的的越鞠丸组和对照组在不动时间上没有明显差异(P> 0.05)。电生理实验中,Balb/c小鼠的越鞠丸组与对照组相比,场电位斜率百分比显著升高(P <0.01),而C57BL/6J小鼠的越鞠丸组和对照组的场电位斜率百分比无显著性差异(P> 0.05);Balb/c小鼠的越鞠丸组与对照组相比,长时程增强显著升高(P <0.01),而C57BL/6J小鼠的越鞠丸组和对照组的长时程增强无显著性差异(P> 0.05)。结论:越鞠丸可能通过增加Balb/c小鼠前额皮层的场电位及长时程增强以提高突触传递效能,从而产生抗抑郁作用。

This paper aimed at comparing the differences of antidepressant effects of Yueju Pill between the Balb/c mice and C57 BL/6 J mice and analyzing the effects on synaptic transmission in mice prefrontal cortex. Healthy adult Balb/c mice and C57 BL/6 J mice were randomly divided into control group and Yueju Pill group. Control group was treated with saline, Yueju Pill group was treated with single dose of Yueju Pill(13.5 g·kg-1). The forced swimming test(FST) was measured 30 min after administration. The field excitatory postsynaptic potential(fEPSP) and long-term potentiation(LTP) of mice prefrontal cortex were detected by the electrophysiological experiment. In FST, the immobility time of Balb/c mice in Yueju Pill group was significantly lower than that in control group(P < 0.01), and the immobility time of C57 BL/6 J mice showed no remarkable difference between Yueju Pill group and control group(P > 0.05). In electrophysiological experiment, the percentage of slope of fEPSP in Balb/c mice in Yueju Pill group was significantly higher than that in control group(P < 0.01), while there was no significantly difference in C57 BL/6 J mice between Yueju Pill group and control group(P > 0.05). The LTP of Balb/c mice in Yueju Pill group was significantly increased than that in control group(P < 0.01), while there was no significantly difference in LTP of C57 BL/6 J mice between Yueju Pill group and control group(P > 0.05). Yueju Pill may display rapid antidepressant effect via increasing fEPSP and LTP in prefrontal cortex of Balb/c mice and then enhancing the synaptic transmission.