大鼠后肢去负荷体位调节装置设计与实验研究

Design and Experimental Validation of Hindlimb Unloading Rat Suspension Device with Adjustable Body Position

通过对大鼠尾吊模型进行改进,研制出一种新型可调节体位的大鼠后肢去负荷悬吊装置,研究模拟微重力效应下体液分布变化对大鼠骨代谢的影响.将36只SD大鼠均分为对照组(CON)、头低位后肢去负荷组(HDT)、水平位后肢去负荷组(HH)和头高位后肢去负荷组(HUT) 4组,实验21天后,利用DXA检测大鼠的骨密度(BMD).模拟微重力效应下的三组大鼠后肢均发生严重骨丢失,其中HH和HUT组后肢BMD显著大于HDT组.实验结果表明,体液分布变化可能在模拟微重力效应导致的骨丢失中起到重要作用,新型大鼠后肢去负荷悬吊装置能够调节大鼠体位(体液)进行模拟微重力效应研究.

The rat tail-suspension model was improved in this paper to develop a new type of adjustable body position hindlimb unloading suspension device for rats, which was used to investigate the effect of changes in body fluid distribution under simulated microgravity on bone metabolism in rats. 36 Sprague-Dawley rats were randomly divided into four groups: Control(CON) group,Head-Down-Tilt(HDT) group, Head-Horizontal(HH) group and Head-Up-Tilt(HUT) group. 21 days later, rats were detected for Bone Mineral Density(BMD) with DXA. Severe bone loss occurred in all three groups of rats under the simulated microgravity effect, and the hindlimb BMD of HH and HUT groups significantly increased compared with HDT group. The experimental results show that the changes in body fluid distribution may play an important role on the bone loss caused by simulated microgravity effect, and the new rat hindlimb unloading suspension device can adjust the body position(body fluid) of rats for simulated microgravity effect study.