葛枣猕猴桃果糖激酶基因电子克隆和生物信息学分析

In Silico Cloning and Bioinformatics Analysis of Fructokinase Gene from Actinidia Polygama

目的:果糖激酶是促进果糖磷酸化关键代谢步骤重要的酶。通过电子克隆技术对葛枣猕猴桃果糖激酶基因进行预测。方法:以毛花猕猴桃果糖激酶序列为探针,基于美国国立生物技术信息中心(NCBI)中葛枣猕猴桃的EST数据库和CAP3在线软件进行序列拼接,利用生物信息学数据库及相关软件对其结构和功能进行预测分析。结果:葛枣猕猴桃果糖激酶基因全长1 015 bp,包含957 bp的开放阅读框,编码318个氨基酸,该蛋白为疏水性蛋白。结论:本研究为进一步解释葛枣猕猴桃果糖激酶基因的分子功能奠定理论及实验基础。

Objective:Fructokinases is an important enzyme catalyzing the key metabolic step of fructose phosphorylation.To obtain the fructokinase gene from Actinidia polygama by using in silico cloning.Methods:Fructokinases were cloned by ETS database and using the CAP3 bioinformatics software with Actinidia eriantha fructokinase sequence as a querying probe,and bioinformatics database and related software were used to predict the structure and function.Results:The full length of fructokinase gene was 1015 bp and contained a 957 bp ORF with 318 amino acids.The protein was predicted as a hydrophobic protein.Conclusion:The study lays theoretical and experimental basis for further explaining the molecular genetic function of fructokinase.