摘要
Zinc plays a significant role in oxidative balance and central nervous systems. Herein, we reported a highly sensitive fluorescent probe DR, bearing a morpholine group and a BPEN ligand in the naphthalimide fluorophore. Upon Zn2+binding, DR exhibited remarkable fluorescence enhancement, and showed high sensitivity to Zn2+with the association constant of 4.9 ? 108 L/mol, and the detection limit of15 nmol/L. Confocal imaging experiments indicated that DR was able to localize to lysosomes in MCF-7 cells. Moreover, upon H2 O2 stimulation in SH-SY5 Y cells, endogenous release of Zn2+was observed.
Zinc plays a significant role in oxidative balance and central nervous systems. Herein, we reported a highly sensitive fluorescent probe DR, bearing a morpholine group and a BPEN ligand in the naphthalimide fluorophore. Upon Zn^(2+)binding, DR exhibited remarkable fluorescence enhancement, and showed high sensitivity to Zn^(2+)with the association constant of 4.9×10~8 L/mol, and the detection limit of 15nmol/L. Confocal imaging experiments indicated that DR was able to localize to lysosomes in MCF-7 cells. Moreover, upon H_2O_2 stimulation in SH-SY5Y cells, endogenous release of Zn^(2+)was observed.
基金
the National Natural Science Foundation of China (No. 21476077) for financial support
