护肝方919糖浆干预产后束缚应激小鼠对仔代体质量及血浆Leptin的影响

Effects on body mass and blood leptin in offspring after intervention of mice under postpartum immobilization stress with Huganfang 919 syrup

目的研究919糖浆干预产后束缚应激小鼠对仔代体质量及血浆Leptin的影响。方法将产后雌鼠分为对照组、应激组及919糖浆治疗组。各组在束缚应激的同时,919糖浆治疗组每天灌胃给予919糖浆(27 g·kg^(-1)·d^(-1)),应激组给予等剂量的0.9%NaCl溶液,对照组不作任何干预。3周后断乳,观察仔鼠体质量、血浆Leptin及肝脏Ob-Rb mRNA的变化情况。结果与对照组相比,6、13、18、23周应激组与919糖浆治疗组仔鼠均出现低体质量,差异有统计学意义(P<0.05);应激组与919糖浆治疗组比较,仔鼠体质量差异无统计学意义(P>0.05)。23周龄时,应激组仔鼠较对照组血浆Leptin水平提高,919糖浆治疗组较应激组仔鼠血浆Leptin水平下降,差异均有统计学意义(P<0.01)。23周龄时,与对照组比较,应激组仔鼠肝脏Ob-Rb mRNA相对表达水平升高,差异有统计学意义(P<0.01); 919糖浆治疗组较应激组仔鼠肝脏Ob-Rb mRNA表达下降,差异有统计学意义(P<0.05); 3组之间雄性仔代小鼠比较,肝脏Ob-Rb mRNA表达差异无统计学意义(P>0.05)。结论产后束缚应激导致仔代低体质量与Leptin相关,护肝方919糖浆可通过调节肝脏OB-Rb mRNA来改善Leptin的变化。

Objective To study the effects on body mass and blood leptin in offspring after intervention of mice under postpartum immobilization stress with Huganfang 919 syrup. Methods The postpartum female mice were randomly assigned into the control group,the immobilization stress( IS) group and the 919 syrup treatment group. At the time of IS,the 919 syrup treatment group was administered 919 syrup by gastric gavage daily( 27 g·kg^-1·d^-1),the IS group was given an equal dose of 0.9% NaCl solution and the control group received no treatment. At weaning 3 weeks later,changes in the body mass,blood leptin and Ob-Rb mRNA in the liver were observed in the offspring. Results Compared to the control group,decreased body mass was observed in the IS and the 919 syrup treatment groups at 6,13,18 and 23 weeks of age,with statistically significant difference( P<0.05);and there was no statistically significant difference in body mass between the IS and the 919 syrup treatment groups( P>0.05). At 23 weeks of age,the level of plasma leptin increased in the IS group compared to the control group,while it decreased in the 919 syrup treatment group compared to the IS group,both with statistically significant difference( P<0.01). At 23 weeks of age,the relative expression of Ob-Rb mRNA in the liver increased significantly in the IS group compared to the control group,( P<0.01),while it decreased significantly in the 919 syrup treatment group compared to the IS group( P<0.05);and there was no significant difference in the male offspring among the three groups( P>0.05). Conclusion The low body mass caused by postpartum IS is associated with leptin,and Huganfang 919 syrup improves the changes in leptin by regulating OB-Rb mRNA in the liver.