铁线蕨提取物对慢性高原病大鼠模型心脏的保护作用

Protective effect of extract of Adiantum capillus-veneris L. on cardiac tissue of rat model with chronic mountain sickness

目的观察铁线蕨提取物对慢性高原病(CMS)大鼠模型心脏的保护作用。方法将50只SD大鼠随机分为平原对照组(CG)、高原模型组(MG)、硝苯地平阳性对照组(NE)、铁线蕨醇提组(ACVL.AG)和铁线蕨水提组(ACVL.WG),每组10只。其中40只大鼠在CMS模型条件下喂养30 d造模,造模成功后给药15 d。剩余10只在平原条件下喂养45 d。观察各组大鼠肺动脉压和心脏组织形态学的变化并检测大鼠C-反应蛋白(CRP)、红细胞生成素(EPO)、血管内皮生长因子(VEGF)和载脂蛋白A1(Apo-A1)等血清学指标。结果与平原对照组比较,高原模型组、硝苯地平阳性对照组、铁线蕨醇提组和铁线蕨水提组大鼠体内的PAP、CRP、EPO和VEGF水平上升(P<0.05),Apo-A1水平下降(P<0.05),提示高原缺氧环境导致大鼠的心脏组织受到损伤。与高原模型组比较,铁线蕨醇提组和铁线蕨水提组大鼠体内的CRP、EPO和VEGF水平降低(P<0.05),Apo-A1水平升高(P<0.05)。同时,光镜下可见CMS模型大鼠心脏组织发生病理性改变,铁线蕨提取物干预之后心脏组织形态发生显著的改善。结论铁线蕨提取物干预对CMS大鼠模型心脏组织有保护作用。

Objective To observe the protective effect of the extract of Adiantum capillus-veneris L.on cardiac tissue of rat model with chronic mountain sickness(CMS).Method A total of 50 rats were randomly divided into different groups namely plain control group(CG),high altitude model group(MG),nifedipine positive control group(NE),alcohol group of Adiantum capillus-veneris L.(ACVL.AG)and water group of Adiantum capillus-veneris L.(ACVL.WG),with 10 rats in each group.Among them 40 rats were fed for 30 days under the CMS molding condition and then were given the medicine for 15 days after successful molding.The remaining 10 rats were fed under plain condition for 45 days.The changes of pulmonary artery pressure and cardiac histomorphology were observed and the serum indexes such as C-reaction protein(CRP),erythropoietin(EPO),vascular endothelial growth factor(VEGF)and apolipoprotein A1(Apo-A1)were detected in rats.Results Compared with the plain control group,the levels of PAP,CRP,EPO,VEGF in the high altitude model group,nifedipine positive control group,alcohol group of Adiantum capillus-veneris L.and water group of Adiantumcapillus-veneris L.were increased(P<0.05)and level of Apo-A1 was decreased steadily(P<0.05),which suggest that hypoxia at high altitude can damage the heart tissue of rats.Compared with the high altitude model group,the levels of CRP,EPO and VEGF were decreased(P<0.05)and the level of Apo-A1 was increased(P<0.05)in the alcohol group of Adiantum capillus-veneris L.and water group of Adiantum capillus-veneris L..At the same time,pathological changes of heart tissue were observed in CMS model rats under light microscope.The heart histomorphology was significantlyimproved after the intervention of the extract of Adiantum capillus-veneris L..Conclusion The extract of Adiantum capillus-veneris L.has protective effect on the heart of CMS rat model.